Chaudhari K, Chattopadhyay A, Dutta S K
Department of Pathology, University College of Medicine, Calcutta.
Indian J Pathol Microbiol. 2000 Oct;43(4):387-94.
125 formalin fixed human tissues from different organs and 50 fresh animal tissues were taken. Each tissue piece was divided into two. Fresh animal tissues were fixed and processed in a domestic microwave oven and formalin fixed tissue were only processed in microwave oven. Simultaneous conventional processing was also carried out. Among the fresh tissues, 34 pieces were fixed in 10% formalin and 16 were stabilized in normal saline, with microwave irradiation. For histoprocessing graded ethanol (70% and absolute) for 150 tissues and graded isopropanol (70% and absolute) for 25 tissues were used for dehydration in microwave technique. Chloroform for 95 tissues, xylene for 15 tissues and isopropanol for 65 tissues were used as clearing agent in microwave technique. Liquid paraffin was impregnating agent in all 175 cases. The oven was operated at 50% power for 10 cases and 40% power for 165 cases. Recording of temperature could not be done. Regarding fixation with formalin 80% cases gave satisfactory result, while with normal saline, only 30% cases were satisfactory. Regarding dehydration with ethanol 80% were satisfactory and with isopropanol 60% were satisfactory. Regarding clearing--both chloroform and isopropyl alcohol gave satisfactory results in 80% cases but with, xylene tissues were fragmented and brittle.
选取了125份来自不同器官的福尔马林固定的人体组织和50份新鲜动物组织。将每块组织切成两半。新鲜动物组织在国产微波炉中进行固定和处理，福尔马林固定的组织仅在微波炉中处理。同时也进行了常规处理。在新鲜组织中，34块用10%福尔马林固定，16块用生理盐水稳定并进行微波照射。在微波技术中，150份组织用梯度乙醇（70%和无水乙醇）脱水，25份组织用梯度异丙醇（70%和无水异丙醇）脱水。95份组织用氯仿、15份组织用二甲苯、65份组织用异丙醇作为微波技术中的透明剂。所有175例均用液体石蜡作为浸渗剂。10例烤箱以50%功率运行，165例以40%功率运行。无法记录温度。关于福尔马林固定，80%的病例结果令人满意，而用生理盐水固定的病例中，只有30%令人满意。关于乙醇脱水，80%令人满意，异丙醇脱水60%令人满意。关于透明——氯仿和异丙醇在80%的病例中都得到了满意的结果，但用二甲苯处理的组织破碎且易碎。
