Feirabend H K, Ploeger S, Kok P, Choufoer H
Dept. of Physiology, University of Leiden, The Netherlands.
Eur J Morphol. 1992;30(4):312-27.
The question whether or not microwave irradiation exerts other than thermal effects on histological staining is still a matter of controversy. The present study was undertaken to reveal or reject such a so far hypothetical non-thermal irradiation effect. A device was developed, which enables exposure of histological sections or tissue pieces to microwave irradiation under isothermal conditions, i.e. with synchronous removal of the internal heat produced. Three classical neuroanatomical staining methods were tested on human and rat CNS. As control, identical procedures were performed without simultaneous microwave irradiation. The experiments were performed at three different temperature levels ranging from 5 to 50 degrees C. In none of the cases studied was a light microscopically appreciable difference observed between the microwave and non-microwave versions of a stain at the same temperature. The hypothesis of a separate non-thermal effect of microwave irradiation on histological staining is therefore rejected.
微波辐射对组织学染色是否产生除热效应以外的其他影响这一问题仍存在争议。本研究旨在揭示或否定这种迄今为止尚属假设的非热辐射效应。我们开发了一种装置，该装置能够在等温条件下，即同时去除产生的内部热量的情况下，使组织切片或组织块受到微波辐射。我们对人和大鼠的中枢神经系统进行了三种经典神经解剖学染色方法的测试。作为对照，在不进行同步微波辐射的情况下进行相同的操作。实验在5至50摄氏度的三个不同温度水平下进行。在所研究的所有情况下，在相同温度下，染色的微波处理版本和非微波处理版本之间在光学显微镜下均未观察到明显差异。因此，关于微波辐射对组织学染色存在单独非热效应的假设被否定。
