Perales-Vela Hugo Virgilio, González-Moreno Sergio, Montes-Horcasitas Carmen, Cañizares-Villanueva Rosa Olivia
Laboratorio de Biotecnología de Microalgas, Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ave. IPN 2508, San Pedro Zacatenco, C.P. 07360, México DF, Mexico.
Chemosphere. 2007 May;67(11):2274-81. doi: 10.1016/j.chemosphere.2006.11.036. Epub 2007 Jan 30.
In the present paper we investigated the effects of sub-lethal concentrations of Cu2+ in the growth and metabolism of Scenedesmus incrassatulus. We found that the effect of Cu2+ on growth, photosynthetic pigments (chlorophylls and carotenoids) and metabolism do not follow the same pattern. Photosynthesis was more sensitive than respiration. The analysis of chlorophyll a fluorescence transient shows that the effect of sub-lethal Cu2+ concentration in vivo, causes a reduction of the active PSII reaction centers and the primary charge separation, decreasing the quantum yield of PSII, the electron transport rate and the photosynthetic O2 evolution. The order of sensitivity found was: Growth>photosynthetic pigments content=photosynthetic O2 evolution>photosynthetic electron transport>respiration. The uncoupled relationship between growth and metabolism is discussed.
在本论文中，我们研究了亚致死浓度的Cu2+对斜生栅藻生长和代谢的影响。我们发现，Cu2+对生长、光合色素（叶绿素和类胡萝卜素）和代谢的影响并不遵循相同模式。光合作用比呼吸作用更敏感。叶绿素a荧光瞬变分析表明，体内亚致死浓度的Cu2+会导致活性PSII反应中心和原初电荷分离减少，降低PSII的量子产率、电子传递速率和光合放氧量。发现的敏感性顺序为：生长>光合色素含量=光合放氧量>光合电子传递>呼吸作用。本文还讨论了生长与代谢之间的解偶联关系。
