Gea S, Gruppi A, Basso B, Menso E, Vottero-Cima E
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
J Clin Lab Immunol. 1990 Apr;31(4):183-7.
The expression of T. cruzi electronegative antigens (FIV) on the parasite surface and their cross-reactivity with heart tissue antigens was studied. For the former purpose epimastigotes (EPI) treated with glutaraldehyde were used to absorb antibodies against surface antigens. Glutaraldehyde fixed heart tissue was used for absorption of antibodies in sera from two groups of chagasic patients with normal and altered electrocardiogram. The absorption of sera from normal electrocardiogram group with EPI significantly reduced the anti FIV activity by ELISA (p less than 0.001). The decreased reactivity was observed with the antigenic bands focused at pI about 4.5. Thus, the results indicate that chagasic patients without electrocardiographic alterations have a high percentage of antibodies reactive with T. cruzi cell surface antigens. Serum absorption with glutaraldehyde fixed heart tissue reduced the anti FIV activity from both groups of patients by ELISA and diminished the intensity of several bands focused at pI 4-6.
研究了克氏锥虫阴性电抗原（FIV）在寄生虫表面的表达及其与心脏组织抗原的交叉反应性。为了实现前一个目的，使用经戊二醛处理的无鞭毛体（EPI）来吸收针对表面抗原的抗体。戊二醛固定的心脏组织用于吸收两组心电图正常和异常的恰加斯病患者血清中的抗体。用EPI吸收心电图正常组的血清，通过酶联免疫吸附测定（ELISA）显著降低了抗FIV活性（p小于0.001）。在聚焦于约4.5的等电点的抗原条带处观察到反应性降低。因此，结果表明，无心电图改变的恰加斯病患者中有高比例的抗体与克氏锥虫细胞表面抗原反应。用戊二醛固定的心脏组织进行血清吸收，通过ELISA降低了两组患者的抗FIV活性，并减弱了聚焦于4 - 6等电点的几条条带的强度。
