Hawkins Kenneth R, Steedman Mark R, Baldwin Richard R, Fu Elain, Ghosal Sandip, Yager Paul
Department of Bioengineering, University of Washington, Seattle, WA, USA.
Lab Chip. 2007 Feb;7(2):281-5. doi: 10.1039/b612894g. Epub 2006 Dec 18.
We present a method for characterizing the adsorption of solutes in microfluidic devices that is sensitive to both long-lived and transient adsorption and can be applied to a variety of realistic device materials, designs, fabrication methods, and operational parameters. We have characterized the adsorption of two highly adsorbing molecules (FITC-labeled bovine serum albumin (BSA) and rhodamine B) and compared these results to two low adsorbing species of similar molecular weights (FITC-labeled dextran and fluorescein). We have also validated our method by demonstrating that two well-known non-fouling strategies [deposition of the polyethylene oxide (PEO)-like surface coating created by radio-frequency glow discharge plasma deposition (RF-GDPD) of tetraethylene glycol dimethyl ether (tetraglyme, CH(3)O(CH(2)CH(2)O)(4)CH(3)), and blocking with unlabeled BSA] eliminate the characteristic BSA adsorption behavior observed otherwise.
我们提出了一种用于表征微流控设备中溶质吸附的方法，该方法对长寿命吸附和瞬态吸附均敏感，并且可应用于各种实际的设备材料、设计、制造方法和操作参数。我们已经表征了两种高吸附性分子（异硫氰酸荧光素标记的牛血清白蛋白（BSA）和罗丹明B）的吸附情况，并将这些结果与两种分子量相似的低吸附性物质（异硫氰酸荧光素标记的葡聚糖和荧光素）进行了比较。我们还通过证明两种著名的抗污染策略[由四甘醇二甲醚（四甘醇，CH(3)O(CH(2)CH(2)O)(4)CH(3)）的射频辉光放电等离子体沉积（RF-GDPD）产生的聚环氧乙烷（PEO）类表面涂层的沉积，以及用未标记的BSA进行封闭]消除了否则会观察到的特征性BSA吸附行为，从而验证了我们的方法。
