Salim Malinda, Mishra Gautam, Fowler Gregory J S, O'sullivan Brian, Wright Phillip C, McArthur Sally L
Department of Chemical and Process Engineering, University of Sheffield, Mappin Street, Sheffield, UKS1 3JD.
Lab Chip. 2007 Apr;7(4):523-5. doi: 10.1039/b615328c. Epub 2007 Feb 14.
This Technical Note presents the direct surface modification of a glass/PTFE hybrid microfluidic chip, via radio frequency glow discharge plasma polymerisation of tetraethlylene glycol dimethylether (tetraglyme), to produce hydrophilic, non-fouling, PEO-like surfaces. We use several techniques including X-ray photoelectron spectroscopy (XPS), direct enzyme-linked immunosorbent assays (ELISA) and immunofluorescent imaging to investigate the channel coatings. Our results indicate the successful deposition of a PEO-like coating onto microchannel surfaces that has both solution and shelf stability (>3 months) and is capable of preventing fibrinogen adsorption to the microchannel surfaces.
本技术说明介绍了通过四甘醇二甲醚（四甘醇二甲醚）的射频辉光放电等离子体聚合对玻璃/聚四氟乙烯混合微流控芯片进行直接表面改性，以制备亲水性、无污垢、类似聚环氧乙烷的表面。我们使用了多种技术，包括X射线光电子能谱（XPS）、直接酶联免疫吸附测定（ELISA）和免疫荧光成像来研究通道涂层。我们的结果表明，已成功在微通道表面沉积了类似聚环氧乙烷的涂层，该涂层具有溶液稳定性和储存稳定性（>3个月），并且能够防止纤维蛋白原吸附到微通道表面。
