Maehle L, Metcalf R A, Ryberg D, Bennett W P, Harris C C, Haugen A
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
Cancer Res. 1992 Jan 1;52(1):218-21.
The carcinogenicity of certain nickel compounds is well known. We have previously shown that human kidney epithelial cells were immortalized by treatment with Ni(II) and in cooperation with the v-Ha-ras oncogene transformed the cells to acquire tumorigenicity in athymic nude mice. Immunocytochemistry and sequence analysis of DNA from the nickel-immortalized cells revealed abnormal p53 expression and a T----C transition mutation in codon 238. These data are consistent with the hypothesis that Ni(II)-induced mutation in the p53 gene can be involved in the escape from senescence of kidney epithelial cells.
某些镍化合物的致癌性是众所周知的。我们之前已经表明，人肾上皮细胞经Ni(II)处理后永生化，并与v-Ha-ras癌基因协同作用，使细胞在无胸腺裸鼠中获得致瘤性。对镍永生化细胞的免疫细胞化学和DNA序列分析显示，p53表达异常，且密码子238处发生T----C转换突变。这些数据与以下假设一致，即p53基因中的Ni(II)诱导突变可能参与了肾上皮细胞衰老逃逸过程。
