Tveito G, Hansteen I L, Dalen H, Haugen A
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
Cancer Res. 1989 Apr 1;49(7):1829-35.
The occupational and environmental hazards of nickel exposure are of great concern in environmental medicine. Nickel workers have increased risk of cancer of the nose, lung, larynx, and possibly the kidney. In the present investigation we have studied the effects of nickel ions on fetal human kidney cortex explants. The explants were continuously exposed to 5 micrograms/ml NiSO4. After 70-100 days in culture foci of phenotypically altered cells appeared. Immortalized cell lines were established and demonstrated to be of human epithelial origin. Tumorigenicity was not induced, but the cells demonstrated decreased requirement for serum, increased plating efficiency and saturation density, and formation of colonies in soft agar. Chromosome changes in the treated cells were observed. Worth mentioning are change in ploidy (3n) and abnormalities of chromosomes 1, 7, 9, 11, 13, 14 and 20; increased numbers of chromosome 17; and loss of normal chromosomes 20 and 22.
在环境医学领域，镍暴露所带来的职业和环境危害备受关注。镍作业工人患鼻癌、肺癌、喉癌以及可能的肾癌风险增加。在本研究中，我们研究了镍离子对人胎儿肾皮质外植体的影响。将外植体持续暴露于5微克/毫升的硫酸镍中。培养70 - 100天后，出现了表型改变的细胞灶。建立了永生化细胞系，并证明其起源于人类上皮细胞。未诱导出肿瘤形成，但这些细胞表现出对血清需求降低、接种效率和饱和密度增加以及在软琼脂中形成集落。观察到处理后细胞的染色体变化。值得一提的是多倍体变化（3n）以及1号、7号、9号、11号、13号、14号和20号染色体异常；17号染色体数量增加；以及正常的20号和22号染色体缺失。
