聚集性脑细胞培养物中氟化3,4 - 二羟基苯丙氨酸异构体的比较代谢

Comparative metabolism of fluorinated 3,4-dihydroxyphenylalanine isomers in aggregating brain cell cultures.

作者信息

Wiese C, Cogoli-Greuter M, Weinreich R, Winterhalter K H

机构信息

Paul-Scherrer-Institut, Villigen-PSI, Zürich, Switzerland.

出版信息

J Neurochem. 1992 Jan;58(1):219-26. doi: 10.1111/j.1471-4159.1992.tb09299.x.

DOI:10.1111/j.1471-4159.1992.tb09299.x

PMID:1727432

Abstract

Fluorinated analogues of 3,4-dihydroxyphenylalanine (DOPA) were tested for intracellular metabolic conversion in aggregating cell cultures prepared from fetal rat brain. 5-Fluoro-D/L-DOPA was methylated almost exclusively to 3-O-methyl-5-fluoro-D/L-DOPA. Metabolism of 6-fluoro-D/L-DOPA resulted in 6-fluorodopamine, 6-fluoro-3,4-dihydroxyphenylacetic acid, and 3-O-methyl-6-fluoro-D/L-DOPA, but with a qualitatively and quantitatively different metabolite pattern compared with that of L-DOPA and D/L-DOPA, respectively. Homovanillic acid and fluorohomovanillic acid have not been found intracellularly in the cultures. On the basis of these data, the model development of the cerebral metabolism of tracers used in positron emission tomography can be improved.

摘要

在由胎鼠脑制备的聚集细胞培养物中，对3,4 - 二羟基苯丙氨酸（多巴）的氟化类似物进行了细胞内代谢转化测试。5 - 氟 - D/L - 多巴几乎完全甲基化为3 - O - 甲基 - 5 - 氟 - D/L - 多巴。6 - 氟 - D/L - 多巴的代谢产物包括6 - 氟多巴胺、6 - 氟 - 3,4 - 二羟基苯乙酸和3 - O - 甲基 - 6 - 氟 - D/L - 多巴，但与L - 多巴和D/L - 多巴的代谢产物模式相比，在定性和定量上均有所不同。在培养物的细胞内未发现高香草酸和氟高香草酸。基于这些数据，可以改进正电子发射断层扫描中使用的示踪剂的脑代谢模型开发。