Reynhout Irene C, Cornelissen Jeroen J L M, Nolte Roeland J M
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
J Am Chem Soc. 2007 Feb 28;129(8):2327-32. doi: 10.1021/ja066790f. Epub 2007 Feb 3.
The synthesis and self-assembly behavior of biohybrid ABC triblock copolymers consisting of a synthetic diblock, polystyrene-b-polyethylene glycol (PSm-b-PEG113), where m is varied, and a hemeprotein, myoglobin (Mb) or horse radish peroxidase (HRP), is described. The synthetic diblock copolymer is first functionalized with the heme cofactor and subsequently reconstituted with the apoprotein or the apoenzyme to yield the protein-containing ABC triblock copolymer. The obtained amphiphilic block copolymers self-assemble in aqueous solution into a large variety of aggregate structures. Depending on the protein and the polystyrene block length, micellar rods, vesicles, toroids, figure eight structures, octopus structures, and spheres with a lamellar surface are formed.
描述了由合成二嵌段聚苯乙烯-b-聚乙二醇（PSm-b-PEG113，其中m可变）和血蛋白、肌红蛋白（Mb）或辣根过氧化物酶（HRP）组成的生物杂交ABC三嵌段共聚物的合成及自组装行为。首先用血红素辅因子对合成二嵌段共聚物进行功能化，随后用脱辅基蛋白或脱辅酶进行重构，以得到含蛋白质的ABC三嵌段共聚物。所得到的两亲性嵌段共聚物在水溶液中自组装成多种聚集体结构。根据蛋白质和聚苯乙烯嵌段长度的不同，会形成胶束棒、囊泡、环面、数字8结构、章鱼结构以及具有层状表面的球体。
