用于冷冻电子显微镜的冷冻水合生物标本的聚焦离子束减薄
Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy.
作者信息
Marko Michael, Hsieh Chyongere, Schalek Richard, Frank Joachim, Mannella Carmen
机构信息
Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, Albany, New York 12201, USA.
出版信息
Nat Methods. 2007 Mar;4(3):215-7. doi: 10.1038/nmeth1014. Epub 2007 Feb 4.
Cryo-electron microscopy can provide high-resolution structural information about cells and organelles in the nearly native, frozen-hydrated state. Applicability, however, is limited by difficulties encountered in preparing suitably thin, vitreously frozen biological specimens. We demonstrate, by cryo-electron tomography of Escherichia coli cells, that a focused ion beam (FIB) can be used to thin whole frozen-hydrated cells in a convenient and essentially artifact-free way.
冷冻电子显微镜可以在接近天然的冷冻水合状态下提供有关细胞和细胞器的高分辨率结构信息。然而，其适用性受到制备适当薄的、玻璃化冷冻生物标本时遇到的困难的限制。我们通过对大肠杆菌细胞进行冷冻电子断层扫描证明，聚焦离子束（FIB）可用于以方便且基本无伪像的方式使整个冷冻水合细胞变薄。
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