Majumder Parijat, Zhang Peijun
Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK.
Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 0DE, UK; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7BN, UK.
Curr Opin Struct Biol. 2025 Aug;93:103076. doi: 10.1016/j.sbi.2025.103076. Epub 2025 Jun 4.
As cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) continue to advance, the ability to visualize cellular and organismal structures with unprecedented clarity is redefining the landscape of structural biology. Breakthroughs in imaging technology, sample preparation and image processing now enable the detailed elucidation of cellular architecture, macromolecular organization, and dynamic biological processes at sub-nanometer resolution. Recent methodological advances have propelled the field to new frontiers, facilitating the investigation of complex biological questions across scales-from macromolecular complexes to organism-wide structural insights. This review explores rapidly emerging trends, highlights key innovations that are pushing the boundaries of in situ structural biology, and addresses persistent challenges in expanding the applicability of cryo-EM and cryo-ET across diverse biological systems.
随着冷冻电子显微镜（cryo-EM）和冷冻电子断层扫描（cryo-ET）技术的不断进步，以前所未有的清晰度可视化细胞和生物体结构的能力正在重新定义结构生物学的格局。成像技术、样品制备和图像处理方面的突破现在能够在亚纳米分辨率下详细阐明细胞结构、大分子组织和动态生物学过程。最近的方法学进展将该领域推向了新的前沿，促进了跨尺度复杂生物学问题的研究——从大分子复合物到全生物体的结构洞察。本综述探讨了迅速出现的趋势，强调了推动原位结构生物学边界的关键创新，并讨论了在将冷冻电子显微镜和冷冻电子断层扫描技术应用于各种生物系统时所面临的持续挑战。
