Zelenin A V, Kirianova E A, Nesterova M V, Kolesnikov V A, Severin E S
Mol Biol Rep. 1975 Oct;2(3):241-5. doi: 10.1007/BF00356994.
The influence of a specific histone kinase, phosphorylating lysine-rich histone F1, F2a2, F2b, on the physico-chemical properties of the chromatin in the whole undestroyed fixed cell, has been investigated. It was found that the exogenous histone kinase penetrates into the nuclei of the undestroyed fixed cells and into the isolated unfixed nuclei and changes the physico-chemical properties of the chromatin there, bringing about an increase in binding of a basic dye acridine orange and a decrease in its stability to heat.
研究了一种特异性组蛋白激酶对完整未破坏固定细胞中染色质物理化学性质的影响，该激酶可使富含赖氨酸的组蛋白F1、F2a2、F2b磷酸化。结果发现，外源性组蛋白激酶可穿透完整未破坏固定细胞的细胞核以及分离出的未固定细胞核，并改变其中染色质的物理化学性质，导致碱性染料吖啶橙的结合增加，且其对热的稳定性降低。
