控制金纳米棒的细胞摄取。
Controlling the cellular uptake of gold nanorods.
作者信息
Huff Terry B, Hansen Matthew N, Zhao Yan, Cheng Ji-Xin, Wei Alexander
机构信息
Department of Chemistry and Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Langmuir. 2007 Feb 13;23(4):1596-9. doi: 10.1021/la062642r.
Abstract
Gold nanorods coated with cetyltrimethylammonium bromide (CTAB), a cationic micellar surfactant used in nanorod synthesis, were rapidly and irreversibly internalized by KB cells via a nonspecific uptake mechanism. Internalized nanorods near the cell surface were monitored by two-photon luminescence (TPL) microscopy and observed to migrate toward the nucleus with a quadratic rate of diffusion. The internalized nanorods were not excreted but formed permanent aggregates within the cells, which remained healthy and grew to confluence over a 5-day period. Nonspecific nanorod uptake could be greatly reduced by displacing the CTAB surfactant layer with chemisorptive surfactants, particularly by the conjugation of poly(ethylene glycol) chains onto nanorods using in situ dithiocarbamate formation.
摘要
包覆有十六烷基三甲基溴化铵（CTAB，一种用于纳米棒合成的阳离子胶束表面活性剂）的金纳米棒通过非特异性摄取机制被KB细胞快速且不可逆地内化。通过双光子发光（TPL）显微镜监测细胞表面附近内化的纳米棒，并观察到它们以二次扩散速率向细胞核迁移。内化的纳米棒没有被排出，而是在细胞内形成了永久性聚集体，细胞在此后5天内保持健康并生长至汇合状态。通过用化学吸附性表面活性剂取代CTAB表面活性剂层，尤其是通过原位形成二硫代氨基甲酸盐将聚（乙二醇）链缀合到纳米棒上，可以大大减少纳米棒的非特异性摄取。
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