Li Yixia, Si Jianhao, Fan Haiyan, Yang Jinxian, Ye Xiaodong
Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China Hefei Anhui 230026 China
CAS Key Laboratory of Soft Matter Chemistry, University of Science and Technology of China Hefei Anhui 230026 China.
RSC Adv. 2018 Aug 2;8(48):27546-27555. doi: 10.1039/c8ra03545h. eCollection 2018 Jul 30.
Reduction-responsive polymer micelles are highly promising drug carriers with better tumor therapeutic effect, which can be achieved by controlled drug release under stimulation. Gold nanorods (AuNRs) have attracted considerable attention due to their unique optical and electronic properties when used for biomedical applications. Herein, the lipoic-acid-functionalized reduction-responsive amphiphilic copolymer poly(ε-caprolactone)--poly[(oligoethylene glycol) acrylate] (LA-PCL-SS-POEGA) with a disulfide group between the two blocks was prepared to modify AuNRs Au-S bonds. The size and morphology of AuNRs@LA-PCL-SS-POEGA were measured by dynamic laser light scattering (DLS) and transmission electron microscopy (TEM) methods. The stabilities of AuNRs@LA-PCL-SS-POEGA in different types of media were studied by UV/vis spectroscopy and DLS techniques. The results show that AuNRs@LA-PCL-SS-POEGA gradually aggregate in a concentrated salt solution containing 150 mM dithiothreitol (DTT), but exhibit high stability in a non-reducing environment. Near infrared (NIR)-induced heating of AuNRs@LA-PCL-SS-POEGA was investigated in an aqueous solution under NIR laser irradiation (808 nm), revealing that AuNRs@LA-PCL-R-POEGA maintain excellent photothermal conversion efficiency after modification. When compared with non-reduction responsive AuNRs@LA-PCL-CC-POEGA, the internalization of AuNRs@LA-PCL-SS-POEGA demonstrates that the reduction-responsive polymer could enhance the cellular uptake of nanoparticles measured by inductively coupled plasma mass spectrometry (ICP-MS) and TEM.
还原响应性聚合物胶束是极具前景的药物载体，具有更好的肿瘤治疗效果，可通过刺激下的可控药物释放来实现。金纳米棒（AuNRs）因其在生物医学应用中独特的光学和电子特性而备受关注。在此，制备了在两个嵌段之间带有二硫键的硫辛酸功能化还原响应性两亲共聚物聚（ε-己内酯）-聚[（聚乙二醇）丙烯酸酯]（LA-PCL-SS-POEGA），用于通过Au-S键修饰AuNRs。通过动态激光散射（DLS）和透射电子显微镜（TEM）方法测量了AuNRs@LA-PCL-SS-POEGA的尺寸和形态。通过紫外/可见光谱和DLS技术研究了AuNRs@LA-PCL-SS-POEGA在不同类型介质中的稳定性。结果表明，AuNRs@LA-PCL-SS-POEGA在含有150 mM二硫苏糖醇（DTT）的浓盐溶液中逐渐聚集，但在非还原环境中表现出高稳定性。在近红外（NIR）激光照射（808 nm）下，研究了AuNRs@LA-PCL-SS-POEGA在水溶液中的近红外诱导加热，结果表明修饰后的AuNRs@LA-PCL-R-POEGA保持了优异的光热转换效率。与非还原响应性AuNRs@LA-PCL-CC-POEGA相比，AuNRs@LA-PCL-SS-POEGA的内化表明，通过电感耦合等离子体质谱（ICP-MS）和TEM测量，还原响应性聚合物可以增强纳米颗粒的细胞摄取。
