Aulitzky W K, Schlegel P N, Wu D F, Cheng C Y, Chen C L, Li P S, Goldstein M, Reidenberg M, Bardin C W
Population Council, Center for Biomedical Research, New York, New York 10021.
Proc Soc Exp Biol Med. 1992 Jan;199(1):93-6. doi: 10.3181/00379727-199-43335.
Measurements of tissue immunoassayable clusterin, a protein associated with programmed cell death and tissue reorganization, were performed in rats treated with nephrotoxic doses of gentamicin sulfate. Adult Lewis rats were treated with 100 mg/kg/day of gentamicin sulfate for 12 days. Urine, serum, and tissue levels of clusterin protein were measured, as were urinary N-acetyl beta-glucosaminidase (NAG) and serum creatinine levels. Induction of renal injury by gentamicin was detectable within 4 days by increased levels of urinary N-acetyl beta-glucosaminidase (from 280 +/- 66 (mean +/- SD) to 910 +/- 210 nmol/mg creatinine), and within 9 days of initiating gentamicin treatment by increased serum creatinine (from 0.5 +/- 0.1 to 1.2 +/- 0.4 mg/dl). Paralleling these changes, renal, urinary, and serum levels of clusterin increased 10-, 116-, and 3-fold (P less than 0.05). Treatment with gentamicin sulfate did not increase clusterin levels in the seminal vesicle, ventral prostate, testis, or epididymis. The measurement of urinary or serum clusterin may play a role in the early detection of renal injury.
对用肾毒性剂量硫酸庆大霉素处理的大鼠进行了组织免疫可检测的聚集素测量，聚集素是一种与程序性细胞死亡和组织重塑相关的蛋白质。成年Lewis大鼠用100mg/kg/天的硫酸庆大霉素处理12天。测量了尿液、血清和组织中聚集素蛋白的水平，以及尿N-乙酰-β-氨基葡萄糖苷酶（NAG）和血清肌酐水平。通过尿N-乙酰-β-氨基葡萄糖苷酶水平升高（从280±66（平均值±标准差）升至910±210nmol/mg肌酐），在4天内可检测到硫酸庆大霉素诱导的肾损伤，在开始硫酸庆大霉素治疗9天内可通过血清肌酐升高（从0.5±0.1升至1.2±0.4mg/dl）检测到。与这些变化平行，肾脏、尿液和血清中的聚集素水平分别升高了10倍、116倍和3倍（P<0.05）。硫酸庆大霉素处理未增加精囊、腹侧前列腺、睾丸或附睾中的聚集素水平。尿或血清聚集素的测量可能在肾损伤的早期检测中发挥作用。
