Chu T T, Fink M Y, Mong J A, John G, Auger A P, Ge Y, Sealfon S C
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, NY 10029, USA.
J Neuroendocrinol. 2007 Mar;19(3):145-61. doi: 10.1111/j.1365-2826.2006.01523.x.
The development of microarray technology makes it possible to simultaneously assay the expression level of hundreds to tens of thousands of mRNA transcripts in one experiment. Genome-wide transcriptional analysis has increasing importance for many areas of neuroendocrinology research. The expense and technical complexity of microarray experiments can make it difficult to navigate the terrain of rival platforms and technologies. In this review, we provide a practical view and comparison of various microarray technologies. Affymetrix arrays, high-density cDNA arrays, membrane arrays and experimental design and data analysis are all discussed by researchers currently using these techniques to study gene regulation in neuroendocrine tissues.
微阵列技术的发展使得在一次实验中同时检测数百至数万个mRNA转录本的表达水平成为可能。全基因组转录分析在神经内分泌学研究的许多领域中日益重要。微阵列实验的成本和技术复杂性可能会使在众多竞争平台和技术中做出选择变得困难。在这篇综述中，我们对各种微阵列技术提供了实用的观点和比较。目前使用这些技术研究神经内分泌组织中基因调控的研究人员对Affymetrix阵列、高密度cDNA阵列、膜阵列以及实验设计和数据分析都进行了讨论。
