通过不同的吡啶二羧酸释放方法检测好氧颗粒中的芽孢
Spore detection in aerobic granules by different dipicolinic acid releasing methods.
作者信息
Wang L, Lin Y M
机构信息
Environmental Science and Engineering College, Ocean University of China, 266100 Qingdao, China.
出版信息
Bioresour Technol. 2007 Nov;98(16):3164-7. doi: 10.1016/j.biortech.2006.10.036. Epub 2007 Feb 5.
Abstract
This work aimed at developing a procedure for spore quantification. Spore content was determined by analyzing dipicolinic acid (dpa) extracted from aerobic granules by 13 methods. Concentrated HCl was able to release dpa completely. Results showed that dpa constituted 33.7 mg per g SS, meaning that about 337 mg per g SS were spores, not the normal vegetative cells.
摘要
这项工作旨在开发一种孢子定量程序。通过13种方法分析从好氧颗粒中提取的吡啶二羧酸（dpa）来确定孢子含量。浓盐酸能够完全释放dpa。结果表明，每克悬浮固体（SS）中dpa含量为33.7毫克，这意味着每克SS中约有337毫克是孢子，而非正常的营养细胞。
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