Galanina L A, Marchenko I V, Skvortsova E K, Kazanskaia T B, Bekhtereva M N
Mikrobiologiia. 1976 May-Jun;45:515-9.
The sublethal dose of calcium hypochlorite (CH) of 0.2--0.3 mg/ml active chlorine did not cause, after 5 min, morphological changes in the spores of Bacillus anthracoides which could be detected by phase contrast microscopy, or a decrease in the content of dipicolinic acid (DPA) in the spores. Further cultivation of the spores treated with the sublethal dose of CH om MPA resulted in a delay of changes which were typical of normal germination process (swelling, loss of light refraction, decrease in DPA content). The action of the lethal dose of CH (0.2--0.3 mg/ml active chlorine during 1.5 hr or 5.6 mg/ml active chlorine during 1 hr) causes a decrease in light refraction, changes in the dimensions of spores, and a decrease in the content of DPA in the spores by a factor of 4--5. A sharp decrease in the content of DPA in the spores may characterize not only their germination but also their death caused by lethal doses of the chlorine containing disinfectant.
次氯酸钙（CH）活性氯含量为0.2 - 0.3毫克/毫升的亚致死剂量，在5分钟后，通过相差显微镜未检测到炭疽芽孢杆菌孢子的形态变化，也未导致孢子中吡啶二羧酸（DPA）含量降低。用CH的亚致死剂量处理过的孢子在MPA上进一步培养，导致正常萌发过程中典型变化（肿胀、失去光折射、DPA含量降低）出现延迟。CH致死剂量（1.5小时内活性氯含量为0.2 - 0.3毫克/毫升或1小时内活性氯含量为5.6毫克/毫升）的作用会导致光折射降低、孢子尺寸变化以及孢子中DPA含量降低4 - 5倍。孢子中DPA含量的急剧降低不仅可能表征其萌发，还可能表征由含氯消毒剂致死剂量导致的死亡。
