Avon Sylvie Louise, Goulet Jean-Paul, Deslauriers Noella
Faculty of Dentistry, Laval University, Ste-Foy, Quebec, Canada.
J Prosthet Dent. 2007 Jan;97(1):32-8. doi: 10.1016/j.prosdent.2006.12.001.
The adhesion of microorganisms to a denture surface can result in denture stomatitis.
The purpose of this study was to evaluate denture biofilm in vivo using a new sampling system to gain a better understanding of plaque organization in Candida-induced denture stomatitis. Removable acrylic resin disks were used with self-adhesive paper disks to compare biofilm formation and retention efficacy of both sampling systems.
Twenty-one subjects with a complete maxillary denture were recruited, 12 with a clinical diagnosis of chronic denture stomatitis and 9 with a clinically healthy palatal mucosa. A custom-made autopolymerized 3.0-mm-diameter acrylic resin disk inlay was inserted in a cavity prepared within the intaglio surface of the maxillary denture in contact with either normal or inflamed mucosa. Next to the acrylic resin disk, a self-adhesive paper disk was placed for comparison of the 2 sampling techniques. Denture biofilms were collected within variable time periods of 1 hour to several months, and descriptive data were provided by means of scanning electron microscopy analyses. The method of retention and recovery of specimen disks were evaluated between both sampling techniques using a chi-square test to assess significant differences (alpha=.05).
Early event features of denture biofilms showed differences in biofilm organization between the 2 sampling techniques but became subtler with increased time periods. Regardless of the sampling technique, interparticipant variations could be observed as to rate, microorganism density, and architectural pattern. In general, denture biofilm formation was increased when the sampling disks were localized in areas affected by denture stomatitis.
The new sampling technique provides reliable information with controlled formation time of denture biofilm in its natural environment.
微生物附着于义齿表面可导致义齿性口炎。
本研究的目的是使用一种新的采样系统在体内评估义齿生物膜，以更好地了解念珠菌性义齿性口炎中菌斑的组织情况。使用可摘丙烯酸树脂盘和自粘纸盘来比较两种采样系统的生物膜形成和保留效果。
招募了21名佩戴上颌全口义齿的受试者，其中12名临床诊断为慢性义齿性口炎，9名腭黏膜临床健康。将定制的直径3.0毫米的自凝丙烯酸树脂盘嵌体插入在上颌义齿组织面制备的与正常或发炎黏膜接触的窝洞内。在丙烯酸树脂盘旁边放置一个自粘纸盘，以比较两种采样技术。在1小时至数月的不同时间段内收集义齿生物膜，并通过扫描电子显微镜分析提供描述性数据。使用卡方检验评估两种采样技术之间标本盘的保留和回收方法，以评估显著差异（α = 0.05）。
义齿生物膜的早期事件特征显示两种采样技术在生物膜组织方面存在差异，但随着时间的增加差异变得更细微。无论采用何种采样技术，在速率、微生物密度和结构模式方面都可观察到个体间的差异。一般来说，当采样盘位于受义齿性口炎影响的区域时，义齿生物膜形成增加。
新的采样技术在自然环境中义齿生物膜形成时间可控的情况下提供了可靠信息。
