Zheng Siyang, Tai Yu-Chong, Kasdan Harvey
Caltech Micromachining Lab, California Institute of Technology, USA.
Conf Proc IEEE Eng Med Biol Soc. 2005;2006:1024-7. doi: 10.1109/IEMBS.2005.1616592.
We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 μm lateral separation was achieved for 5 μm and 10 μm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.
我们报道了一种用于通过白细胞（WBC，即白血球）和红细胞（RBC，即红血球）的大小对其进行连续二元分离的微机电系统（MEMS）装置。该系统由载玻片上流体腔内的聚二甲基硅氧烷（PDMS）圆柱形支柱组成。对于5μm和10μm的珠子，实现了420μm的横向分离。通过模拟成功预测了分离的临界粒径，并通过用聚苯乙烯珠子进行校准实验确定了该粒径。还通过实验测量了分离函数（横向位移与粒径的关系）。使用该装置对稀释的全血和浓缩白细胞的血液组分进行了测试，红细胞与白细胞的比例与血细胞计数结果一致。对荧光标记白细胞的实验证实了白细胞与红细胞的分离，并测量了分离效率。
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