中性粒细胞对于缺血再灌注肺损伤并非必需。

Neutrophils are not necessary for ischemia-reperfusion lung injury.

作者信息

Steimle C N, Guynn T P, Morganroth M L, Bolling S F, Carr K, Deeb G M

机构信息

Department of Thoracic Surgery, University of Michigan Hospitals, Ann Arbor.

出版信息

Ann Thorac Surg. 1992 Jan;53(1):64-72; discussion 72-3. doi: 10.1016/0003-4975(92)90758-v.

DOI:10.1016/0003-4975(92)90758-v

PMID:1728243

Abstract

The role of neutrophils (PMNs) in ischemia-reperfusion injury after lung transplantation is unclear. If PMNs are involved in ischemia-reperfusion injury in the intact rat, then PMNs should sequester in the injured lung and PMN-depleted rats should develop less injury. Group A rats were treated with a rabbit anti-rat PMN antibody causing profound neutropenia (less than 100 PMNs/microL) and group B with control serum (greater than 2,000 PMNs/microL). Rats were anesthetized and left lung ischemia was sustained for 90 or 180 minutes by clamping the bronchus and the pulmonary artery and vein. Lung injury was quantified by the accumulation of radiolabeled (125I) albumin in ischemic left and nonischemic right lungs (cpm per gram of lung/cpm per gram of blood). Ischemia caused significant lung injury (p less than 0.05) in both PMN-depleted (albumin leak index: 90 min, 0.208; 180 min, 0.218) and nondepleted (90 min, 0.222; 180 min, 0.241) animals compared with nonischemic controls (depleted: 90 min, 0.050; 180 min, 0.100; nondepleted: 90 min, 0.063; 180 min, 0.101); microscopy also demonstrated lung injury. The injury was not associated with PMN sequestration as shown by light microscopy. Thus, we conclude that PMNs are not necessary for ischemia-reperfusion injury and PMN-depletion does not attenuate ischemia-reperfusion injury.

摘要

中性粒细胞（PMN）在肺移植后缺血再灌注损伤中的作用尚不清楚。如果PMN参与了完整大鼠的缺血再灌注损伤，那么PMN应在受损肺中滞留，且PMN耗竭的大鼠损伤应较轻。A组大鼠用兔抗大鼠PMN抗体处理，导致严重的中性粒细胞减少（每微升少于100个PMN），B组用对照血清处理（每微升大于2000个PMN）。大鼠麻醉后，通过钳夹支气管、肺动脉和肺静脉使左肺缺血持续90或180分钟。通过放射性标记（125I）白蛋白在缺血的左肺和未缺血的右肺中的积聚来量化肺损伤（每克肺的计数/每克血液的计数）。与非缺血对照组相比，缺血在PMN耗竭（白蛋白渗漏指数：90分钟，0.208；180分钟，0.218）和未耗竭（90分钟，0.222；180分钟，0.241）的动物中均导致显著的肺损伤（p小于0.05）（耗竭组：90分钟，0.050；180分钟，0.100；未耗竭组：90分钟，0.063；180分钟，0.101）；显微镜检查也显示了肺损伤。如光学显微镜所示，损伤与PMN滞留无关。因此，我们得出结论，PMN对于缺血再灌注损伤并非必需，且PMN耗竭不会减轻缺血再灌注损伤。