Wang Cun-Fang, Wu Chang-Xin, Li Ning
College of Animal Science and Technology, China Agricultural University, Beijing, China.
Yi Chuan. 2007 Jan;29(1):75-80. doi: 10.1360/yc-007-0075.
Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor first discovered in cancer cells under hypoxic conditions, and was demonstrated to play a key role in the organism's adaptation to changing oxygen tensions. The Tibetan chicken, a breed indigenous to the Tibetan plateau is particularly adaptable to the low oxygen tension and low temperature conditions in the high altitude environment. By comparison, the White Leghorn and ShouGuang chicken are low-altitude chicken breeds. The eggs of these three chicken breeds were incubated in a fitted hatcher that simulated hypoxic condition. The results demonstrated that the hatching rate for Tibetan chicken was significantly higher than the two low altitude breeds, and Tibetan chicken displayed higher endurance in the hypoxia environment. To a certain degree, the hypoxic condition proved fatal to hatching for the low-altitude chickens. Gene expression of HIF-1alpha was detected in brain and skeletal muscle tissues for three chicken varieties using the TaqMan probe FQRT-PCR method. The results showed that HIF-1alpha mRNA displayed tissue specific differential expression, with the highest in the brain. In addition, the expression of HIF-1alpha mRNA in the brain of Tibetan chicken embryos was similar to that of the low-altitude chickens when they were hatched under normal oxygen tensions.
缺氧诱导因子-1（HIF-1）是一种转录因子，最初在缺氧条件下的癌细胞中被发现，并被证明在生物体适应不断变化的氧张力过程中起关键作用。藏鸡是原产于青藏高原的一个品种，特别适应高海拔环境中的低氧张力和低温条件。相比之下，白来航鸡和寿光鸡是低海拔鸡种。将这三个鸡种的蛋在模拟缺氧条件的合适孵化器中孵化。结果表明，藏鸡的孵化率显著高于两个低海拔鸡种，并且藏鸡在缺氧环境中表现出更高的耐受性。在一定程度上，缺氧条件对低海拔鸡种的孵化是致命的。使用TaqMan探针FQRT-PCR方法检测了三个鸡种的脑和骨骼肌组织中HIF-1α的基因表达。结果表明，HIF-1α mRNA表现出组织特异性差异表达，在脑中最高。此外，当藏鸡胚胎在正常氧张力下孵化时，其脑中HIF-1α mRNA的表达与低海拔鸡种相似。
