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鸡中醛缩酶C(ALDOC)的差异基因表达与低氧适应

Differential gene expression of aldolase C (ALDOC) and hypoxic adaptation in chickens.

作者信息

Wang C F, Yuan C Z, Wang S H, Zhang H, Hu X X, Zhang L, Wu C, Li N

机构信息

College of Animal Science and Technology, China Agricultural University, Beijing, 100094, China.

出版信息

Anim Genet. 2007 Jun;38(3):203-10. doi: 10.1111/j.1365-2052.2007.01605.x.

Abstract

Two sequence variants of the aldolase C (ALDOC) gene were discovered based on comparison of the sequences from an altiplano chicken breed (Tibetan chicken) and two lowland breeds (White Leghorn and ShouGuang). Gel-shift results indicated that one of these variants, L25375:c.310-258G>A, was able to bind hypoxia-induced factor-l (HIF1A), therefore, functioning as a hypoxia response element (HRE). The combined activity of the HRE and HIF1A could increase under the influence of a hypoxic stimulus. Hypoxia leads to increased death rates of chicken embryos, while the L25375:c.310-258G>A described herein is prevalent in healthy embryos grown under hypoxic conditions. Fluorescence quantitative real-time PCR results revealed that HIF1A upregulated the transcript level of the glycolytic enzyme ALDOC in the brain and skeletal muscle of animals subjected to hypoxia. Thus, a large amount of ATP is produced by increased glycolysis, allowing the organism to meet energy metabolism demands. As such, we believe this sequence variant is an adaptation to external anoxic environment.

摘要

基于高原鸡品种(藏鸡)与两个低地鸡品种(白来航鸡和寿光鸡)序列的比较,发现了醛缩酶C(ALDOC)基因的两个序列变体。凝胶迁移结果表明,其中一个变体L25375:c.310 - 258G>A能够结合缺氧诱导因子-1(HIF1A),因此,起到缺氧反应元件(HRE)的作用。在缺氧刺激的影响下,HRE和HIF1A的联合活性会增加。缺氧会导致鸡胚死亡率增加,而本文所述的L25375:c.310 - 258G>A在缺氧条件下生长的健康胚胎中普遍存在。荧光定量实时PCR结果显示,HIF1A上调了缺氧动物大脑和骨骼肌中糖酵解酶ALDOC的转录水平。因此,通过增加糖酵解产生大量ATP,使机体能够满足能量代谢需求。因此,我们认为这种序列变体是对外部缺氧环境的一种适应。

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