Xu Jing, Wang Wei, Chai Bao-Feng, Liang Ai-Hua
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, China.
Yi Chuan. 2007 Jan;29(1):87-91. doi: 10.1360/yc-007-0087.
The expansion of trinucleotide repeats in genome is related to the phthogenesis of several neurodegenerative diseases. A GARP (glutamic acid-rich protein) gene was isolated from the macronuclear plasmid mini library of Euplotes octocarinatus. A micronuclear version of the GARP gene was amplified by polymerase chain reaction. The macronuclear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuclear chromosomes of hypotrichous ciliates. One of the three cysteines is encoded by the opal codon TGA(88-90). The predicted open reading frame encodes a 112-amino acid polypeptide, with a predicted molecular mass of 13 kDa and an isoelectric point of 3.82. Micronuclear version of the GARP gene contains two internal eliminated sequences (IES), IES1 and IES2. IES1 is 41 bp long and is flanked by 5'-GA-3' direct repeats. IES2 is 41 bp long and flanked by 5'-TA-3' direct repeats. Transcriptional activity of GARP gene was confirmed by reverse transcription polymerase chain reaction (RT-PCR).
基因组中三核苷酸重复序列的扩增与几种神经退行性疾病的发病机制有关。从八肋游仆虫的大核质粒小文库中分离出一个富含谷氨酸蛋白（GARP）基因。通过聚合酶链反应扩增出该GARP基因的微核版本。携带GARP基因的大核DNA分子长460 bp，具有下毛目纤毛虫大核染色体的特征。三个半胱氨酸之一由乳白密码子TGA（88 - 90）编码。预测的开放阅读框编码一个112个氨基酸的多肽，预测分子量为13 kDa，等电点为3.82。GARP基因的微核版本包含两个内部消除序列（IES），即IES1和IES2。IES1长41 bp，两侧为5'-GA-3'正向重复序列。IES2长41 bp，两侧为5'-TA-3'正向重复序列。通过逆转录聚合酶链反应（RT-PCR）证实了GARP基因的转录活性。
