The macronuclear gamma-tubulin-encoding gene of Euplotes octocarinatus contains two introns and an in-frame TGA.

The gamma-tubulin (gamma-Tub)-encoding gene (gamma-tub) of Euplotes octocarinatus was amplified from macronuclear DNA with the help of the polymerase chain reaction (PCR) and sequenced. The polypeptide deduced from the gene consists of 462 amino acids (aa). It shares 61% aa identity with the Aspergillus nidulans gamma-Tub. The gene contains an in-frame TGA codon and two small pre-mRNA introns (36 and 26 bp). We suggest that the TGA, like TGA codons in the pheromone-encoding genes of E. octocarinatus, codes for a cysteine. This suggestion is supported by the finding that in the gamma-Tub of other organisms, a cysteine is located at this position. Sequencing the mRNA revealed that the introns are absent from the gamma-tub transcripts. The second intron constitutes the shortest one reported so far. We have also sequenced the 5'- and 3'-flanking regions of the gene up to the telomeres and report here the entire sequence of the macronuclear DNA molecule carrying gamma-tub.