Kongrit Darika, Jisaka Mitsuo, Iwanaga Chitose, Yokomichi Hiroshi, Katsube Takuya, Nishimura Kohji, Nagaya Tsutomu, Yokota Kazushige
Division of Applied Resources Chemistry, The United Graduate School of Agricultural Sciences, Tottori University, Koyama-minami, Tottori, Japan.
Biosci Biotechnol Biochem. 2007 Feb;71(2):491-8. doi: 10.1271/bbb.60537. Epub 2007 Feb 7.
A plant allene oxide synthase (AOS) reacting with 13S-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT), a lipoxygenase product of alpha-linolenic acid, provides an allene oxide which functions as an intermediate for jasmonic acid (JA) synthesis, making AOS a key enzyme regulating the JA level in plants. Although AOSs in various plants have been investigated, there is only limited information about AOSs in soybean (Glycine max). In this study, we cloned and characterized two soybean AOSs, GmAOS1 and GmAOS2, sharing 95% homology in the predicted amino acid sequences. GmAOS1 and GmAOS2 were composed of 564 and 559 amino acids respectively, with predicted N-terminal chloroplast-targeting signal peptides. Both AOSs expressed in Escherichia coli were selective for 13S-hydroperoxides of alpha-linolenic and linoleic acids, suggesting the potential of GmAOS1 and GmAOS2 to contribute to JA synthesis. GmAOS1 and GmAOS2 were expressed in leaves, stems, and roots, suggesting broad distribution in a soybean plant.
一种植物丙二烯氧化物合酶（AOS）与α-亚麻酸的脂氧合酶产物13S-氢过氧-9Z，11E，15Z-十八碳三烯酸（13-HPOT）反应，生成一种丙二烯氧化物，该丙二烯氧化物作为茉莉酸（JA）合成的中间体，使AOS成为调节植物中JA水平的关键酶。尽管已经对各种植物中的AOS进行了研究，但关于大豆（Glycine max）中AOS的信息却非常有限。在本研究中，我们克隆并鉴定了两个大豆AOS，即GmAOS1和GmAOS2，它们在预测的氨基酸序列中具有95%的同源性。GmAOS1和GmAOS2分别由564和559个氨基酸组成，带有预测的N端叶绿体靶向信号肽。在大肠杆菌中表达的两种AOS对α-亚麻酸和亚油酸的13S-氢过氧化物具有选择性，这表明GmAOS1和GmAOS2具有促进JA合成的潜力。GmAOS1和GmAOS2在叶、茎和根中均有表达，表明它们在大豆植株中分布广泛。
