Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Mol Biol Rep. 2012 Mar;39(3):2267-74. doi: 10.1007/s11033-011-0976-y. Epub 2011 Jun 4.
Allene oxide synthase (AOS) is the first committed step in the biosynthetic pathway of Jasmonate. In this study, a full-length cDNA of AOS gene (named as AaAOS) was cloned from Artemisia annua. The gene was 1891 bp in size containing an open reading frame (1581 bp) encoding 526 amino acids. Comparative and bioinformatic analysis revealed that the deduced protein of AaAOS was highly homologous to AOSs from other plant species. Phylogenetic analysis indicated that the protein of AaAOS belonged to the dicotyledonous group, which was consistent with the category of A. annua. Southern blot analysis revealed that it was a low-copy gene. Quantitative Real-time PCR (qRT-PCR) analysis showed that AaAOS mRNA accumulated most abundantly in leaves and flowers. The qRT-PCR analysis revealed that MeJA, ABA and ethylene treatments significantly enhanced AaAOS transcript expression.
烯氧合酶（AOS）是茉莉酸生物合成途径中的第一步。本研究从黄花蒿中克隆得到了 AOS 基因的全长 cDNA（命名为 AaAOS）。该基因大小为 1891bp，包含一个开放阅读框（1581bp），编码 526 个氨基酸。比较和生物信息学分析表明，AaAOS 的推导蛋白与其他植物物种的 AOS 高度同源。系统发育分析表明，AaAOS 蛋白属于双子叶植物组，与黄花蒿的类别一致。Southern blot 分析表明它是一个低拷贝基因。定量实时 PCR（qRT-PCR）分析表明，AaAOS mRNA 在叶片和花朵中积累最多。qRT-PCR 分析显示，MeJA、ABA 和乙烯处理显著增强了 AaAOS 转录本的表达。
