感染大蜡螟的游离多角体病毒的纯化
Purification of free polyhedrosis virus infecting Galleria mellonella L.
作者信息
Strokovskaya L I, Skuratovskaya I N, Zherebtsova E N, Sutugina L P
出版信息
Acta Virol. 1977 Mar;21(2):157-60.
PMID:17285
Abstract
Free Galleria mellonella nuclear polyhedrosis virus was purified 4000--4500 times by Tween-80 treatment and repeated sucrose density gradient centrifugation, based on the ratio of infectivity to protein content as compared to the starting virus-containing suspension.
摘要
通过吐温-80处理和反复的蔗糖密度梯度离心，基于感染性与蛋白质含量的比率与起始含病毒悬浮液相比，将游离的大蜡螟核型多角体病毒纯化了4000至4500倍。
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