感染大蜡螟的核型多角体病毒非封闭颗粒：体内和体外滴定。简要报告。
Non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L.: titration in vivo and in vitro. Brief report.
作者信息
Komissarenko S V, Zherebtsova E N, Sutugina L P, Primatchenko L V
出版信息
Arch Virol. 1979;61(4):347-9. doi: 10.1007/BF01315023.
PMID:391180
Abstract
Infectivity of non-occluded particles of nuclear polyhedrosis virus infecting Galleria mellonella L. was determined with bioassay technique in vivo and with plaque assay method in vitro using SCLd 135 (Quiot) established cell culture. A plaque-forming virus unit corresponds to about 50 ID50 for Galleria larvae injected into hemocoel for non-cloned and 0.1-1.0 ID50 for a virus isolate cloned from an infective plaque.
摘要
利用生物测定技术在体内以及使用已建立的SCLd 135（Quiot）细胞培养物通过噬斑测定法在体外确定了感染大蜡螟（Galleria mellonella L.）的核型多角体病毒非包涵体颗粒的感染性。对于注入血腔的大蜡螟幼虫，一个噬斑形成病毒单位对应于约50个半数感染剂量（ID50）（针对未克隆的病毒），而对于从感染性噬斑克隆的病毒分离株，则对应于0.1 - 1.0个ID50。
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