D'Inca R, Ernst P, Hunt R H, Perdue M H
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
Dig Dis Sci. 1992 Jan;37(1):33-9. doi: 10.1007/BF01308339.
To determine the role of T cells versus mast cells in mucosal injury, we documented structural and functional changes in the intestine of congenitally athymic nude rats during infection with the enteric parasite, Nippostrongylus brasiliensis. Studies were conducted at days 4, 7, 10, and 21 postinfection; controls were uninfected. Villus damage was indicated by morphological abnormalities at days 7, 10, and 21 and reduced activities of disaccharidase enzymes at days 10 and 21. The activity of the proliferative enzyme, thymidine kinase, was increased only at day 21, at which time the crypts were elongated. Epithelial permeability increased significantly: 5-hr recovery (in urine and blood) of the probe molecule, [51Cr]EDTA, following injection into ligated jejunal segments, was elevated at days 7 and 10. Uptake of a protein antigen, ovalbumin, from lumen to blood followed a similar pattern. No evidence of functional T cells was demonstrated. However, mucosal mast-cell activation was indicated by elevated serum levels of rat mast-cell protease II at days 7 and 10. We conclude that the absence of thymus-derived T cells does not preclude mucosal damage involving impaired barrier and digestive function. Mucosal mast cells may be involved in causing the injury in this model.
为了确定T细胞与肥大细胞在粘膜损伤中的作用，我们记录了先天性无胸腺裸鼠在感染肠道寄生虫巴西日圆线虫期间肠道的结构和功能变化。在感染后第4、7、10和21天进行研究；对照组未感染。在第7、10和21天，绒毛损伤表现为形态异常，在第10和21天，二糖酶活性降低。增殖酶胸苷激酶的活性仅在第21天增加，此时隐窝伸长。上皮通透性显著增加：在结扎的空肠段注射探针分子[51Cr]EDTA后，其在尿液和血液中的5小时回收率在第7和10天升高。蛋白质抗原卵清蛋白从肠腔到血液的摄取遵循类似模式。未发现功能性T细胞的证据。然而，在第7和10天，大鼠肥大细胞蛋白酶II的血清水平升高表明粘膜肥大细胞被激活。我们得出结论，胸腺来源的T细胞缺失并不排除涉及屏障和消化功能受损的粘膜损伤。在这个模型中，粘膜肥大细胞可能参与了损伤的发生。
