Larkan Nicholas J, Smith Sally E, Barker Susan J
School of Plant Biology M084, Centre for Soil-Plant Interactions, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
Centre for Soil-Plant Interactions, Perth, Australia.
Mycorrhiza. 2007 Jun;17(4):311-318. doi: 10.1007/s00572-007-0106-9. Epub 2007 Feb 7.
Our research aims to investigate the molecular communication between land plants and arbuscular mycorrhizal (AM) fungi in the establishment of symbiosis. We have identified a mutation in the facultative AM host tomato, which we named rmc. Plants that are homozygous for rmc no longer host most AM fungi. The mutation also affects the interaction of tomato with root knot nematode and Fusarium wilt. However, the function/s encoded by the intact Rmc locus is/are unknown. To clone and sequence the gene or genes that comprise the Rmc locus, we have initiated a positional cloning project. In this paper, we report the construction of mapping populations and use of molecular markers from the published genome map to identify the location of Rmc on tomato chromosome 8. Nucleotide binding site-leucine rich repeat resistance genes, reported to reside in the same region of that chromosome, provided insufficient differences to develop cleaved amplified polymorphic sequence markers. Therefore, we were unable to map these sequences in relation to rmc. Our results potentiate future work to identify the Rmc function and to determine the genetic basis for the multiple plant-microbe interaction functions that the rmc mutation has defined.
我们的研究旨在探究陆地植物与丛枝菌根（AM）真菌在共生建立过程中的分子通讯。我们在兼性AM宿主番茄中鉴定出一个突变，将其命名为rmc。rmc纯合的植物不再能容纳大多数AM真菌。该突变还影响番茄与根结线虫和枯萎病的相互作用。然而，完整的Rmc基因座所编码的功能尚不清楚。为了克隆并测序构成Rmc基因座的一个或多个基因，我们启动了一个定位克隆项目。在本文中，我们报告了定位群体的构建以及利用已发表的基因组图谱中的分子标记来确定Rmc在番茄8号染色体上的位置。据报道位于该染色体同一区域的核苷酸结合位点-富含亮氨酸重复序列抗性基因，差异不足以开发酶切扩增多态性序列标记。因此，我们无法将这些序列与rmc进行定位。我们的结果为未来确定Rmc功能以及确定rmc突变所定义的多种植物-微生物相互作用功能的遗传基础的工作提供了助力。
