Kim Byong-Chul, Kang Tae-Wook, Kim Moo-Sang, Kim Chang-Bae
National Genome Information Centre, 52 Eoun-dong, Yusong-gu, Daejeon, South Korea.
DNA Seq. 2006 Jun;17(3):181-6. doi: 10.1080/10425170600806928.
The complete nucleotide sequence of the mitochondrial genome from the R. uyekii with a total size of 16,817 bp has been determined by long PCR technology. Mitogenome of R. uyekii encoding 13 putative proteins, two ribosomal RNAs and 22 tRNAs shows typical teleost mitogenome structure. Nucleotide composition, amino acid composition and codon usage are in the range of values estimated from other teleost mitogenomes. In the AT rich region of R. uyekii, several conserved blocks which are identified from vertebrates are observed in the genome. R. uyekii, the Korean endemic species, belongs to cyprinid fish from which the information of nine mitogenomes is available. To understand the phylogenetic relationships of Cypriniformes from the known mitogenome information, we analysed Cypriniformes mitogenome based on protein coding gene sequences. In spite of more resolved picture of phylogenetic interrelationships in cyprinid fish in this study, the further study with comprehensive taxon sampling for mitogenome information is strongly needed.
利用长PCR技术测定了乌氏红鲌线粒体基因组的完整核苷酸序列,其总大小为16,817 bp。乌氏红鲌的线粒体基因组编码13种推定蛋白、2种核糖体RNA和22种tRNA,呈现出典型的硬骨鱼线粒体基因组结构。核苷酸组成、氨基酸组成和密码子使用情况均在从其他硬骨鱼线粒体基因组估计的值范围内。在乌氏红鲌富含AT的区域,在基因组中观察到几个从脊椎动物中鉴定出的保守区域。乌氏红鲌是韩国特有物种,属于鲤科鱼类,已有9个线粒体基因组的信息。为了从已知的线粒体基因组信息了解鲤形目鱼类的系统发育关系,我们基于蛋白质编码基因序列分析了鲤形目鱼类的线粒体基因组。尽管本研究中鲤科鱼类的系统发育相互关系有了更清晰的图景,但仍强烈需要对线粒体基因组信息进行全面分类群采样的进一步研究。