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须鱲卵黄蛋白原Ao1基因的特征、表达谱及启动子分析

Characterization, expression profile, and promoter analysis of the Rhodeus uyekii vitellogenin Ao1 gene.

作者信息

Kong Hee Jeong, Kim Ju Lan, Moon Ji Young, Kim Woo-Jin, Kim Hyung Soo, Park Jung Youn, Cho Hyun Kook, An Cheul Min

机构信息

Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea.

Department of Molecular Biology, Pusan National University, Busan 609-735, Korea.

出版信息

Int J Mol Sci. 2014 Oct 17;15(10):18804-18. doi: 10.3390/ijms151018804.

DOI:10.3390/ijms151018804
PMID:25329620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4227248/
Abstract

The fish Vitellogenin (Vg) gene has been applied as a biomarker for exposure to estrogenic compounds in the aquatic environment. In this study, we cloned and characterized Vg cDNA from the Korean rose bitterling Rhodeus uyekii (Ru-Vg). The Ru-Vg cDNA encodes a 1424-amino-acid polypeptide that belongs to the VgAo1 family and contains a putative signal peptide, lipovitellin I, phosvitin, and lipovitellin II, but does not contain the vWFD domain or the C-terminal peptide. The deduced Ru-Vg protein has high amino acid identity (73.97%-32.17%) with fish Vg proteins. Pairwise alignment and phylogenetic analysis revealed that Ru-Vg is most closely related to Acheilognathus yamatsutae Vg. Ru-Vg transcripts were detected using quantitative polymerase chain reaction in all tissues tested, with the highest level of expression observed in the ovary. Ru-Vg mRNA was upregulated in R. uyekii hepatopancreas cells in response to treatment with 17β-estradiol (E2) or 17α-ethinylestradiol (EE2). Luciferase reporter expression, driven by the 5'-regulatory region of the Ru-Vg gene spanning from -1020 bp to the start codon was induced by the estrogen receptor and was synergistically activated by treatment with E2 or EE2. These results suggest that R. uyekii and the Ru-Vg gene may be useful as biomarkers for exposure to E2 or EE2.

摘要

鱼类卵黄蛋白原（Vg）基因已被用作水环境中雌激素化合物暴露的生物标志物。在本研究中，我们从韩国玫瑰无须鲃（Rhodeus uyekii，Ru-Vg）中克隆并鉴定了Vg cDNA。Ru-Vg cDNA编码一个1424个氨基酸的多肽，属于VgAo1家族，包含一个假定的信号肽、卵黄脂磷蛋白I、卵黄高磷蛋白和卵黄脂磷蛋白II，但不包含vWFD结构域或C末端肽。推导的Ru-Vg蛋白与鱼类Vg蛋白具有较高的氨基酸同一性（73.97%-32.17%）。成对排列和系统发育分析表明，Ru-Vg与山女鳟（Acheilognathus yamatsutae）Vg关系最为密切。使用定量聚合酶链反应在所有测试组织中检测到Ru-Vg转录本，在卵巢中观察到最高表达水平。在用17β-雌二醇（E2）或17α-乙炔雌二醇（EE2）处理后，Ru-Vg mRNA在韩国玫瑰无须鲃肝细胞中上调。由Ru-Vg基因5'-调控区（从-1020 bp到起始密码子）驱动的荧光素酶报告基因表达受雌激素受体诱导，并被E2或EE2处理协同激活。这些结果表明，韩国玫瑰无须鲃和Ru-Vg基因可能作为E2或EE2暴露的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/8fc975389653/ijms-15-18804-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/baa7f5ce7fba/ijms-15-18804-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/62d0f9e3269b/ijms-15-18804-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/e9729995cfba/ijms-15-18804-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/0a22766a7110/ijms-15-18804-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/8fc975389653/ijms-15-18804-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/baa7f5ce7fba/ijms-15-18804-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/62d0f9e3269b/ijms-15-18804-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/e9729995cfba/ijms-15-18804-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/922764bd5375/ijms-15-18804-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/0a22766a7110/ijms-15-18804-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bd/4227248/8fc975389653/ijms-15-18804-g006.jpg

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