Wang Binhua, Wang Shanghong, Hu Maolin, He Zhihong, Wang Fang
a Department of Biological Science , College of Life Science, Nanchang University , Nanchang , China and.
b Bureau of Agriculture of the city of Jinggangshan , Jiangxi , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):3880-3881. doi: 10.3109/19401736.2014.987244. Epub 2014 Dec 8.
In this study, the complete mitochondrial genome (mitogenome) sequence of Paracheilognathus imberbis (Cypriniformes: Cyprinidae: Acheilognathinae) was determined by long PCR and primer-walking methods. The complete mitochondrial genome is 16,819 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes as well as a displacement loop (D-loop). The overall base composition of the genome is A(29.73%), T(27.25%), C(26.63%) and G(17.40%), respectively. The mitogenome of P. imberbis displayed novel gene order arrangement compared with published Rhodeus sinensis to date. The mitogenome would contribute to resolving phylogenetic position and interrelationships of Acheilognathinae.
在本研究中，通过长PCR和引物步移法测定了间颌副须鲃（鲤形目：鲤科：副须鲃亚科）的完整线粒体基因组（线粒体基因组）序列。完整的线粒体基因组长度为16,819 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因以及一个位移环（D环）。基因组的总体碱基组成分别为A（29.73%）、T（27.25%）、C（26.63%）和G（17.40%）。与迄今已发表的中华鳑鲏相比，间颌副须鲃的线粒体基因组显示出新颖的基因顺序排列。该线粒体基因组将有助于解决副须鲃亚科的系统发育位置和相互关系。
