Sarret Y, Woodley D T, Grigsby K, Wynn K, O'Keefe E J
Department of Dermatology, Stanford University, CA 94305.
J Invest Dermatol. 1992 Jan;98(1):12-6. doi: 10.1111/1523-1747.ep12493517.
Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) are two powerful mitogens for human keratinocytes that also have been shown to promote the healing of in vivo wounds. Transforming growth factor-beta (TGF-beta) markedly inhibits human keratinocyte proliferation and growth and yet has been shown to promote wound healing. Using a migration assay that evaluates pure cell locomotion independently from cell proliferation, we examined the influence of EGF, bFGF, and TGF-B on human keratinocyte locomotion. Although these agents had profound influences upon the growth potential of keratinocytes in parallel thymidine incorporation assays, they had no significant effect upon keratinocyte locomotion when cells were apposed to either tissue culture plastic or a collagen substratum. In contrast, we found that bovine pituitary extract (BPE), a poorly defined mitogen that is commonly used in keratinocyte cultures, could stimulate keratinocyte locomotion when the cells were apposed to a collagen substrate. These studies demonstrate that i) keratinocyte locomotion and proliferation operate by completely independent mechanisms, ii) the positive effects upon wound healing by EGF, bFGF, and TGF-beta are not due to a direct promotion of keratinocyte locomotion, and iii) that one or more components of BPE are capable of directly promoting keratinocyte locomotion on collagen.
表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）是对人角质形成细胞有强大作用的两种促细胞分裂剂，它们也已被证明可促进体内伤口愈合。转化生长因子-β（TGF-β）显著抑制人角质形成细胞的增殖和生长，但也已被证明可促进伤口愈合。我们使用一种迁移试验来独立于细胞增殖评估纯细胞运动，研究了EGF、bFGF和TGF-β对人角质形成细胞运动的影响。尽管在平行的胸腺嘧啶核苷掺入试验中这些因子对角质形成细胞的生长潜力有深远影响，但当细胞贴附于组织培养塑料或胶原基质时，它们对角质形成细胞运动没有显著影响。相反，我们发现牛垂体提取物（BPE），一种常用于角质形成细胞培养的成分不明确的促细胞分裂剂，当细胞贴附于胶原底物时可刺激角质形成细胞运动。这些研究表明：i）角质形成细胞运动和增殖通过完全独立的机制起作用；ii）EGF、bFGF和TGF-β对伤口愈合的积极作用并非由于直接促进角质形成细胞运动；iii）BPE的一种或多种成分能够直接促进角质形成细胞在胶原上的运动。
