在通过天然化学连接获得的多肽中，在半胱氨酸（Acm）存在的情况下对半胱氨酸进行选择性脱硫。
Selective desulfurization of cysteine in the presence of Cys(Acm) in polypeptides obtained by native chemical ligation.
作者信息
Pentelute Brad L, Kent Stephen B H
机构信息
Department of Chemistry, Institute for Biophysical Dynamics, Gordon Center for Integrative Science, The University of Chicago, Chicago Illinois 60637, USA.
出版信息
Org Lett. 2007 Feb 15;9(4):687-90. doi: 10.1021/ol0630144.
Increased versatility for the synthesis of proteins and peptides by native chemical ligation requires the ability to ligate at positions other than Cys. Here, we report that Raney nickel can be used under standard conditions for the selective desulfurization of Cys in the presence of Cys(Acm). This simple and practical tactic enables the more common Xaa-Ala junctions to be used as ligation sites for the chemical synthesis of Cys-containing peptides and proteins. [reaction: see text].
通过天然化学连接合成蛋白质和肽时，若要提高其通用性，则需要能够在除半胱氨酸（Cys）以外的位置进行连接。在此，我们报告，在标准条件下，阮内镍可用于在存在Cys（Acm）的情况下对Cys进行选择性脱硫。这种简单实用的策略使更常见的Xaa-Ala连接能够用作含Cys肽和蛋白质化学合成的连接位点。[反应：见正文]
Zotero 插件