Ring Gabriela, Londei Paola, Eichler Jerry
Department of Life Sciences, Ben Gurion University, Beersheva, Israel.
FEMS Microbiol Lett. 2007 May;270(1):34-41. doi: 10.1111/j.1574-6968.2007.00649.x. Epub 2007 Feb 5.
Translation initiation in Archaea combines aspects of the parallel process in Eukarya and Bacteria alongside traits unique to this domain. To better understand translation initiation in Archaea, an in vitro translation system from the haloarchaeon Haloferax volcanii has been developed. The ability to translate individual mRNAs both under the conditions used in previously developed poly(U)-dependent poly(Phe) synthesis systems as well as under physiological conditions was shown. Using the H. volcanii system, mRNAs proceeded by either 'strong' or 'weak' Shine-Dalgarno (SD) motifs, or completely lacking leader sequences were effectively translated. The in vitro haloarchaeal system also successfully translated mRNA from Bacteria, again either presenting a SD initiation motif or completely lacking a leader sequence. Thus, the ability to translate individual mRNAs in vitro offers a system to address translation initiation as well as other aspects of protein biogenesis in Archaea.
古菌中的翻译起始过程融合了真核生物和细菌中平行过程的多个方面，同时还具备该领域独有的特征。为了更好地理解古菌中的翻译起始过程，人们开发了一种来自嗜盐古菌沃氏嗜盐菌（Haloferax volcanii）的体外翻译系统。该系统展示了在先前开发的依赖多聚尿苷酸（poly(U)）的多聚苯丙氨酸（poly(Phe)）合成系统所使用的条件下，以及在生理条件下翻译单个信使核糖核酸（mRNA）的能力。利用沃氏嗜盐菌系统，具有“强”或“弱”夏因-达尔加诺（Shine-Dalgarno，SD）基序的mRNA，或者完全缺乏前导序列的mRNA都能被有效翻译。体外嗜盐古菌系统还成功翻译了来自细菌的mRNA，这些mRNA同样要么具有SD起始基序，要么完全缺乏前导序列。因此，体外翻译单个mRNA的能力为研究古菌中的翻译起始以及蛋白质生物合成的其他方面提供了一个系统。
