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P2X7受体表达水平决定了嘌呤类危险信号在T淋巴细胞中的致死效应。

P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes.

作者信息

Aswad Fred, Dennert Gunther

机构信息

Department of Molecular Microbiology, USC/Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA.

出版信息

Cell Immunol. 2006 Sep;243(1):58-65. doi: 10.1016/j.cellimm.2006.12.003. Epub 2007 Feb 6.

DOI:10.1016/j.cellimm.2006.12.003
PMID:17286969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1913480/
Abstract

Contact of T lymphocytes with nicotinamide adenine dinucleotide (NAD) or ATP causes cell death that requires expression of purinergic receptor P2X(7) (P2X(7)R). T cell subsets differ in their responses to NAD and ATP, which awaits a mechanistic explanation. Here, we show that sensitivity to ATP correlates with P2X(7)R expression levels in CD4 cells, CD8 cells and CD4(+)CD25(+) cells from both C57BL/6 and BALB/c mice. But P2X(7)R ligands do not only induce cell death but also shedding of CD62L. It is shown here that in CD62L(high) T cells, CD62L shedding correlates with low expression of P2X(7)Rs and lower cell death, whereas in CD62L(low) cells P2X(7)R expression and death are higher. The possibility is therefore investigated that P2X(7)Rs induce T cell activation. Experiments show that spontaneous T cell proliferation is somewhat higher in cells expressing P2X(7)Rs, but this effect we suggest is caused by P2X(7)R expression on accessory cells.

摘要

T淋巴细胞与烟酰胺腺嘌呤二核苷酸（NAD）或三磷酸腺苷（ATP）接触会导致细胞死亡，这种细胞死亡需要嘌呤能受体P2X（7）（P2X(7)R）的表达。T细胞亚群对NAD和ATP的反应不同，这有待于作出机制上的解释。在此，我们表明，来自C57BL/6和BALB/c小鼠的CD4细胞、CD8细胞和CD4(+)CD25(+)细胞对ATP的敏感性与P2X(7)R表达水平相关。但是P2X(7)R配体不仅诱导细胞死亡，还会导致CD62L的脱落。本文表明，在CD62L(高)的T细胞中，CD62L的脱落与P2X(7)Rs的低表达及较低的细胞死亡相关，而在CD62L(低)的细胞中，P2X(7)R的表达和细胞死亡更高。因此，研究了P2X(7)Rs诱导T细胞活化的可能性。实验表明，在表达P2X(7)Rs的细胞中，自发的T细胞增殖略高，但我们认为这种效应是由辅助细胞上的P2X(7)R表达引起的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/1913480/d2fe7836f129/nihms19492f6.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/1913480/d2fe7836f129/nihms19492f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/1913480/2ba5456fd252/nihms19492f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/1913480/d524790478e6/nihms19492f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33e2/1913480/d2fe7836f129/nihms19492f6.jpg

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