Huang Rui, Mu Xiaojing, Yin Yongguang, Wei Weili, Chen Zhitao, Xia Zhining
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.
Se Pu. 2006 Nov;24(6):597-600. doi: 10.1016/s1872-2059(06)60025-4.
On the basis of non-aqueous capillary electrophoresis (NACE) and micellar electrokinetic chromatography (MEKC), a novel technique, non-aqueous micellar electrokinetic chromatography (NAMEKC), has been established. NAMEKC has the advantages of NACE and uses the separation mechanism of MEKC, showing special advantages for separation of hydrophobic compounds. Separation of three of the priority pollutants by U. S. Environmental Protection Agency (EPA), i. e. dimethyl phthalate (DMP), diethyl phthalate (DEP), and dibutyl phthalate (DBP), were realized in 15 min. Important factors on separation, such as the amount of water added in the electrophoretic running buffer, the acidity of water phase, the organic additive, and the concentration of sodium dodecyl sulfate (SDS), were investigated. The proportion of water in the electrophoretic running buffer could affect the current and the stability of SDS micelle. Organic additives and the acidity of water phase showed no effect on increasing resolution. The concentration of SDS was a dominant factor, affecting the partition of analytes in micelle. DMP, DEP, and DBP were separated in a short time under the optimized operation conditions using 20 mmol/L NaH2PO4 and 120 mmol/L SDS in formamide/water (9/1, v/v). The application of NAMEKC leads to successful separation of the three typical hydrophobic compounds, which provides a novel means to separate and analyze hydrophobic compounds.
基于非水毛细管电泳（NACE）和胶束电动色谱（MEKC），建立了一种新技术——非水胶束电动色谱（NAMEKC）。NAMEKC兼具NACE的优点，并采用MEKC的分离机制，在分离疏水性化合物方面显示出独特优势。美国环境保护局（EPA）列出的三种优先污染物，即邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）和邻苯二甲酸二丁酯（DBP），在15分钟内实现了分离。研究了电泳运行缓冲液中添加的水量、水相酸度、有机添加剂以及十二烷基硫酸钠（SDS）浓度等对分离的重要影响因素。电泳运行缓冲液中的水比例会影响电流和SDS胶束的稳定性。有机添加剂和水相酸度对提高分离度无影响。SDS浓度是影响分析物在胶束中分配的主要因素。在优化的操作条件下，使用20 mmol/L NaH2PO4和120 mmol/L SDS的甲酰胺/水（9/1，v/v）体系，可在短时间内分离DMP、DEP和DBP。NAMEKC的应用成功分离了三种典型的疏水性化合物，为疏水性化合物 的分离分析提供了一种新方法。
