Shen Changmao, Duan Wengui, Cen Bo, Tan Jianhui
College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
Se Pu. 2006 Nov;24(6):619-24.
Essential oils were extracted by steam distillation from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm grown in Guangxi. Various factors such as pine needle dosage and extraction time which may influence the oil yield were investigated. The optimum conditions were found to be as follows: pine needle dosage 700 g, extraction time 5 h. The essential oil yields from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm were 0.45% and 0.19%, respectively. Moreover, the chemical compositions of the essential oils were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Sixty four components in the essential oil from needle of Pinus massoniana Lamb were separated and twenty of them (98.59%) were identified while seventy three components in the essential oil from needle of Pinus elliottottii Engelm were separated and twenty nine of them (94.23%) were identified. Generally, the compositions of the essential oils from needles of the two varieties were similar but the contents of some compounds differed greatly. Especially, the content of alpha-pinene in the essential oils from Pinus massoniana Lamb needles was 2.6 times as that from Pinus elliottottii Engelm needles, but the content of beta-pinene was less than the latter. Mono- and sesquiterpenes were the main composition of the essential oils from Pinus massoniana Lamb and Pinus elliottottii Engelm needles.
采用水蒸气蒸馏法从广西种植的马尾松和湿地松针叶中提取挥发油。考察了松针用量、提取时间等影响出油率的各种因素。得出最佳条件如下：松针用量700 g，提取时间5 h。马尾松和湿地松针叶的挥发油得率分别为0.45%和0.19%。此外，采用气相色谱（GC）和气相色谱-质谱联用（GC-MS）对挥发油的化学成分进行了分析。从马尾松针叶挥发油中分离出64个成分，鉴定出其中20个（占98.59%）；从湿地松针叶挥发油中分离出73个成分，鉴定出其中29个（占94.23%）。总体而言，两个品种针叶挥发油的成分相似，但部分化合物的含量差异较大。尤其是，马尾松针叶挥发油中α-蒎烯的含量是湿地松针叶挥发油中α-蒎烯含量的2.6倍，但β-蒎烯的含量低于湿地松针叶挥发油。单萜和倍半萜是马尾松和湿地松针叶挥发油的主要成分。
