乳腺癌脑转移中BCL-2的mRNA和蛋白表达降低，而BAX的mRNA表达降低但蛋白表达增加：一项实时PCR和免疫组化评估。

Reduced mRNA and protein expression of BCL-2 versus decreased mRNA and increased protein expression of BAX in breast cancer brain metastases: a real-time PCR and immunohistochemical evaluation.

作者信息

Stark Andreas M, Pfannenschmidt Saskia, Tscheslog Hauke, Maass Nicolai, Rösel Frank, Mehdorn H Maximilian, Held-Feindt Janka

机构信息

Department of Neurosurgery, University of Schleswig-Holstein Medical Center, Campus Kiel, Kiel, Germany.

出版信息

Neurol Res. 2006 Dec;28(8):787-93. doi: 10.1179/016164106X110364.

DOI:10.1179/016164106X110364

Abstract

OBJECTIVES

Brain metastases are an increasingly common complication in breast cancer patients. Apoptosis regulating genes are promising candidates for further treatment options. We examined the mRNA and protein expression of p53, BCL-2 and BAX in breast cancer brain metastases versus primary tumors.

METHODS

In a two-step approach p53, BCL-2 and BAX mRNA expression in ductal invasive breast cancer brain metastases was examined by: (1) reverse transcription-polymerase chain reaction (RT-PCR) mRNA expression screening (band appearance in relation to an internal standard) and (2) quantitative real-time RT-PCR (CT-values in relation to an internal standard). Protein expression using immunohistochemistry. Results were compared with primary tumors.

RESULTS

We found significantly lower BCL-2 mRNA and protein expression in breast cancer brain metastases versus primary tumors. P53 mRNA and protein expression was also lower in metastases. However, this difference was only significant on mRNA but not on the protein level. BAX expression evaluation revealed was contradictory results: mRNA expression was significantly lower whereas protein expression was significantly higher in metastatic lesions.

DISCUSSION

The mRNA and protein expression of p53 and BCL-2 seems to be reduced in breast cancer brain metastases. BAX mRNA and protein may be regulated differentially in metastatic lesions.

摘要

目的

脑转移是乳腺癌患者中日益常见的并发症。凋亡调节基因是进一步治疗方案的有前景的候选者。我们研究了乳腺癌脑转移灶与原发肿瘤中p53、BCL-2和BAX的mRNA及蛋白表达情况。

方法

采用两步法检测导管浸润性乳腺癌脑转移灶中p53、BCL-2和BAX的mRNA表达：(1)逆转录-聚合酶链反应（RT-PCR）mRNA表达筛查（条带出现情况与内标相关）和(2)定量实时RT-PCR（CT值与内标相关）。采用免疫组织化学检测蛋白表达。将结果与原发肿瘤进行比较。

结果

我们发现乳腺癌脑转移灶中BCL-2的mRNA和蛋白表达明显低于原发肿瘤。转移灶中p53的mRNA和蛋白表达也较低。然而，这种差异仅在mRNA水平上显著，在蛋白水平上不显著。BAX表达评估结果相互矛盾：转移灶中mRNA表达明显较低，而蛋白表达明显较高。

讨论

乳腺癌脑转移灶中p53和BCL-2的mRNA和蛋白表达似乎降低。转移灶中BAX的mRNA和蛋白可能受到不同的调节。