Morikawa Toshie, Takami Toshihiro, Tsuyuguchi Naohiro, Sakamoto Hiroaki, Ohata Kenji, Hara Mitsuhiro
Department of Neurosurgery, Osaka City University Graduate School of Medicine, Osaka, Japan.
Neurol Res. 2006 Dec;28(8):802-6. doi: 10.1179/016164106X110355.
Experimental spinal cord injury (SCI) with focal spinal tissue scarring was studied to better understand the progressive post-traumatic myelomalacia (PPM).
Using a stereotactic device, the authors developed an acute compression of spinal cord at Th-10 in the adult rat. In Group A, the rat thoracic spinal cord was compressed epidurally with preservation of local cerebrospinal fluid (CSF) dynamics. In Group B (spinal tissue scarring), the rat thoracic spinal cord was compressed directly after disruption of meninges of dura mater and arachnoid membrane, followed by gelatine sponge sealing. All rats were maintained for 12 weeks after injury.
Imaging analysis revealed the significant increase of cystic cavitation in the contused spinal cord in Group B compared with Group A. Anterograde axonal tracing demonstrated that the labeled corticospinal axons had extended axonal sprouting into the nearby gray matter and displayed prominent dieback at the rostral interface of the spinal cord lesion in both groups. No significant difference in motor and sensory functions between the two groups was noted.
The new experimental model of PPM formation was devised. Spinal tissue scarring at the injury site may cause a tethering effect on the spinal cord, which may lead to significant alteration of the spinal cord parenchyma. Although the devastating effect of PPM on motor and sensory functions is still not resolved completely, the results in the present study suggest the importance of careful analysis in the treatment of PPM after SCI.
研究伴有局灶性脊髓组织瘢痕形成的实验性脊髓损伤（SCI），以更好地理解创伤后进行性脊髓软化（PPM）。
作者使用立体定向装置在成年大鼠的胸10节段造成脊髓急性压迫。A组大鼠硬膜外压迫胸段脊髓，同时保留局部脑脊液（CSF）动力学。B组（脊髓组织瘢痕形成组）大鼠在硬脑膜和蛛网膜破裂后直接压迫胸段脊髓，随后用明胶海绵封闭。所有大鼠在损伤后维持12周。
影像学分析显示，与A组相比，B组挫伤脊髓中的囊性空洞显著增加。顺行轴突示踪显示，两组中标记的皮质脊髓轴突均有轴突发芽延伸至附近灰质，并在脊髓损伤的头端界面处出现明显的轴突回缩。两组之间的运动和感觉功能无显著差异。
设计了新的PPM形成实验模型。损伤部位的脊髓组织瘢痕形成可能对脊髓产生束缚作用，这可能导致脊髓实质发生显著改变。尽管PPM对运动和感觉功能的破坏性影响仍未完全解决，但本研究结果提示在SCI后PPM治疗中进行仔细分析的重要性。
