Kryzer T C, Gonzalez C, Burgess L P
Department of Surgery, Uniformed Services University of the Health Sciences, Washington, DC.
Ann Otol Rhinol Laryngol. 1992 Jan;101(1):95-9. doi: 10.1177/000348949210100121.
Aerosolized dexamethasone was used in a two-phase study to determine the possible effects on acute subglottic injury in the ferret animal model. In phase 1, equivalent subglottic injuries were made in 10 animals by using the brush technique, and the animals were divided into two groups. The treatment group received aerosolized dexamethasone at 2, 4, and 6 hours postinjury. All animals were examined 2, 4, 6, and 24 hours after the injury. The clinical condition of each animal was evaluated and their airways were measured. The animals were then painlessly killed and the larynges were frozen, sectioned, and photographed at 1-mm intervals. A computer-linked digitizer pad was used to measure the subglottic dimensions. The results show a trend for the treated animals to have a larger subglottic airway as compared to the untreated (control) group. The phase 1 study suggests that there may be an improvement in the subglottic airway when treated acutely with aerosolized dexamethasone. In phase 2, 20 additional animals were studied by using the same methods of injury and treatment as in phase 1. The subglottic airways of these animals were evaluated with histomorphometric analysis on fixed histologic sections. A statistically significant difference was found between the subglottic airways of the treated and untreated animals favoring treatment with aerosolized dexamethasone. Aerosolized dexamethasone appears to be beneficial in preserving the subglottic airway after injury, possibly secondary to decreasing the edema associated with injury.
在一项分两阶段的研究中，使用雾化地塞米松来确定其对雪貂动物模型急性声门下损伤的可能影响。在第一阶段，通过刷涂技术对10只动物造成等效的声门下损伤，并将动物分为两组。治疗组在损伤后2、4和6小时接受雾化地塞米松治疗。所有动物在损伤后2、4、6和24小时接受检查。评估每只动物的临床状况并测量其气道。然后对动物实施无痛安乐死，并将喉部冷冻、切片，每隔1毫米拍照。使用与计算机相连的数字化仪测量声门下尺寸。结果显示，与未治疗（对照）组相比，治疗组动物的声门下气道有增大的趋势。第一阶段研究表明，雾化地塞米松急性治疗时，声门下气道可能会有所改善。在第二阶段，使用与第一阶段相同的损伤和治疗方法，对另外20只动物进行研究。对这些动物的声门下气道在固定组织学切片上进行组织形态计量学分析评估。结果发现，治疗组和未治疗组动物的声门下气道之间存在统计学上的显著差异，雾化地塞米松治疗组更具优势。雾化地塞米松似乎有助于在损伤后保护声门下气道，可能是由于减轻了与损伤相关的水肿。
