腊肠树叶片提取物对二乙基亚硝胺诱导的大鼠肝损伤的影响。
Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats.
作者信息
Pradeep Kannampalli, Mohan Chandrasekaran Victor Raj, Gobianand Kuppannan, Karthikeyan Sivanesan
机构信息
Department of Pharmacology and Environmental Toxicology, Dr. A.L.M. P.G. Institute of Basic Medical Science, University of Madras, Taramani, Chennai 600113, India.
出版信息
Chem Biol Interact. 2007 Apr 5;167(1):12-8. doi: 10.1016/j.cbi.2006.12.011. Epub 2006 Dec 30.
The hepatoprotective and antioxidant effect of Cassia fistula Linn. leaf extract on liver injury induced by diethylnitrosamine (DEN) was investigated. Wistar rats weighing 200+/-10g were administered a single dose of DEN (200mg/kg b.w., i.p.) and left for 30 days. For hepatoprotective studies, ethanolic leaf extract (ELE) of C. fistula Linn. (500mg/kg b.w., p.o.) was administered daily for 30 days. AST, ALT, ALP, LDH, gamma-GT and bilirubin were estimated in serum and liver tissue. Lipid peroxidation (LPO), SOD and CAT were also estimated in liver tissue as markers of oxidative stress. DEN induced hepatotoxicity in all the treated animals were evident by elevated serum ALT, AST, ALP and bilirubin levels and a simultaneous fall in their levels in the liver tissue after 30 days. Induction of oxidative stress in the liver was evidenced by increased LPO and fall in the activities of SOD and CAT. ELE administration for 30 days prevented the DEN induced hepatic injury and oxidative stress. In conclusion, it was observed that ELE of C. fistula Linn. protects the liver against DEN induced hepatic injury in rats.
研究了决明子叶提取物对二乙基亚硝胺（DEN）诱导的肝损伤的保肝和抗氧化作用。将体重为200±10g的Wistar大鼠单次腹腔注射DEN（200mg/kg体重），并饲养30天。为进行保肝研究，每天口服给予决明子乙醇叶提取物（ELE，500mg/kg体重），持续30天。测定血清和肝组织中的AST、ALT、ALP、LDH、γ-GT和胆红素。还测定肝组织中的脂质过氧化（LPO）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）作为氧化应激的标志物。30天后，所有处理动物中DEN诱导的肝毒性表现为血清ALT、AST、ALP和胆红素水平升高，同时肝组织中这些水平下降。肝中氧化应激的诱导表现为LPO增加以及SOD和CAT活性下降。给予ELE 30天可预防DEN诱导的肝损伤和氧化应激。总之，观察到决明子ELE可保护大鼠肝脏免受DEN诱导的肝损伤。
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