Kumar Rajagopal Senthil, Manickam Ponmozhi, Periyasamy Viswanathan, Namasivayam Nalini
Department of Biochemistry, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
J Nutr Biochem. 2003 Aug;14(8):452-8. doi: 10.1016/s0955-2863(03)00053-6.
This study was undertaken to investigate the effect of Cassia auriculata leaf extract on tissue lipid peroxidation and antioxidant status in experimental hepatotoxicity. Administering ethanol to rats for 60 days resulted in significantly elevated levels of serum total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) as compared with those of the experimental control rats. Significantly elevated levels of tissue thiobarbituric acid reactive substances (TBARS), hydroperoxides and lowered activities of superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were also observed on alcohol treatment as compared with those of experimental control rats. Concentration of serum non-enzymic antioxidants such as vitamin E and vitamin C were also significantly lowered on alcohol supplementation. Treatment with Cassia auriculata leaf extract at a dose of 250 mg kg(-1) body weight and 500 mg kg(-1) body weight to rats administered alcohol, lowered the levels of TBARS and hydroperoxides and elevated the activities of SOD and CAT and the levels of reduced GSH in the liver, brain, kidney and intestine significantly compared to unsupplemented alcohol treated rats. Cassia auriculata leaf extract treatment restored the serum vitamin E, and vitamin C levels also to near those of the experimental control animals. Our data indicate that supplementation with Cassia auriculata leaf extract can offer protection against free radical mediated oxidative stress in experimental hepatotoxicity. In addition, histopathological studies of the liver and brain confirmed the beneficial role of Cassia auriculata leaf extract.
本研究旨在探讨耳叶决明叶提取物对实验性肝毒性中组织脂质过氧化和抗氧化状态的影响。与实验对照大鼠相比，给大鼠喂食乙醇60天导致血清总胆红素、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平显著升高。与实验对照大鼠相比，在酒精处理后还观察到组织硫代巴比妥酸反应性物质（TBARS）、氢过氧化物水平显著升高，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性降低以及还原型谷胱甘肽（GSH）水平降低。补充酒精后血清非酶抗氧化剂如维生素E和维生素C的浓度也显著降低。给喂食酒精的大鼠分别以250mg/kg体重和500mg/kg体重的剂量用耳叶决明叶提取物处理，与未补充提取物的酒精处理大鼠相比，显著降低了肝脏、大脑、肾脏和肠道中TBARS和氢过氧化物的水平，提高了SOD和CAT的活性以及还原型GSH的水平。耳叶决明叶提取物处理还使血清维生素E和维生素C水平恢复到接近实验对照动物的水平。我们的数据表明，补充耳叶决明叶提取物可以为实验性肝毒性中自由基介导的氧化应激提供保护。此外，肝脏和大脑的组织病理学研究证实了耳叶决明叶提取物的有益作用。
