酞菁标记寡核苷酸的定量表面增强共振拉曼散射

Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides.

作者信息

Macaskill A, Chernonosov A A, Koval V V, Lukyanets E A, Fedorova O S, Smith W E, Faulds K, Graham D

机构信息

Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.

出版信息

Nucleic Acids Res. 2007;35(6):e42. doi: 10.1093/nar/gkm042. Epub 2007 Feb 8.

DOI:10.1093/nar/gkm042

PMID:17289751

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1874604/

Abstract

The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10(-11) mol. dm(-3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.

摘要

本文报道了用于寡核苷酸表面增强共振拉曼散射（SERRS）检测的酞菁标记物的评估。评估了三种酞菁标记的寡核苷酸，每种都含有不同的金属中心。在可能的情况下，使用两种激发频率确定了每种标记寡核苷酸的检测限。获得了低至2.8×10⁻¹¹ mol·dm⁻³的检测限，这与先前SERRS研究中使用的标准荧光标记探针相当。还展示了无需分离即可鉴定两种酞菁标记的寡核苷酸，表明它们适用于多重检测。这项研究扩展了适用于银纳米颗粒定量表面增强共振拉曼散射的标记物范围，并在考虑将SERRS用于定量DNA检测时提供了更大的灵活性和选择。