Jiang Shou-fang, Yu Li-qun, Leng Shu-guang, Zhang Yan-shu, Cheng Juan, Dai Yu-fei, Niu Yong, He Feng-sheng, Zheng Yu-xin
Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2006 Nov;35(6):675-7.
To investigate the association between polymorphisms of DNA repair gene XRCC1 and DNA damage in peripheral blood lymphocytes among workers exposed to formaldehyde.
One hundred and fifty-one workers exposed to formaldehyde from plywood factories and one hundred and twelve workers without occupational exposure to formaldehyde were recruited into this study. DNA damage levels were measured by comet assay. The polymorphisms of XRCC1 gene were analyzed by polymerase chain reaction(PCR) with restriction fragment length polymorphism(RFLP) method. The multiple covariance analysis was used to compare olive trail moment and comet trail length adjusted confounding factors.
In formaldehyde exposed workers, after ages, smoking and drinking status and occupational exposure level were adjusted, means of Olive trail moment and comet trail length in the subjects with variant genotype at Arg280 His site (geometric means 4.30 and 13.42 respectively) were higher than subjects with wild type homozygote (geometric means 3.38 and 11.71 respectively), the differences were significant (Olive trail moment: P < 0.05, comet trail length: P < 0.01) . No associations between the polymorphisms at other three sites in XRCC1 gene and means of olive trail moment and comet trail length in exposure workers were found.
The polymorphisms of XRCC1 gene may modulate the effects of DNA damages induced by formaldehyde in workers.
探讨甲醛暴露工人DNA修复基因XRCC1多态性与外周血淋巴细胞DNA损伤之间的关联。
本研究招募了151名来自胶合板厂的甲醛暴露工人和112名无职业甲醛暴露的工人。采用彗星试验检测DNA损伤水平。采用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）方法分析XRCC1基因的多态性。运用多协方差分析比较调整混杂因素后的橄榄尾矩和彗星尾长。
在甲醛暴露工人中，调整年龄、吸烟和饮酒状况以及职业暴露水平后，精氨酸280组氨酸位点基因型变异的受试者的橄榄尾矩和彗星尾长均值（几何均值分别为4.30和13.42）高于野生型纯合子受试者（几何均值分别为3.38和11.71），差异具有统计学意义（橄榄尾矩：P < 0.05，彗星尾长：P < 0.01）。未发现XRCC1基因其他三个位点的多态性与暴露工人的橄榄尾矩和彗星尾长均值之间存在关联。
XRCC1基因多态性可能调节甲醛诱导的工人DNA损伤效应。
