Zendehdel Rezvan, Abdolmaleki Parviz, Jouni Fatemeh Javani, Mazinani Mohammad
1 Department of Occupational Hygiene, School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Department of Biophysics, School of Bioscience, Tarbiat Modares University, Tehran, Iran.
Hum Exp Toxicol. 2018 Jul;37(7):690-696. doi: 10.1177/0960327117728385. Epub 2017 Sep 12.
Formaldehyde (FA) has given positive results for the genetic damage evaluated in human peripheral blood lymphocytes. Polymorphism plays a special role in the toxicity of chemicals. DNA damage in blood cells was evaluated in workers who were occupationally exposed to FA. This study tested the association of alcohol dehydrogenase III (ADH3) and manganese-superoxide dismutase (Mn-SOD) gene polymorphism, with DNA damage of peripheral blood lymphocytes. Gene polymorphisms were evaluated in 54 workers from melamine dinnerware workshops and 34 workers from dairy production workshops. The control and exposed population were matched based on age, smoking, work history and socioeconomic status. DNA damage was evaluated by alkaline comet assay in the peripheral blood lymphocytes. ADH3 and Mn-SOD genotypes were determined in all workers using polymerase chain reaction-restriction fragment length polymorphism method. DNA damage of lymphocyte cells was significantly higher ( p < 0.001) in the exposed individuals in comparison with the control population. The examinations of the exposed group had revealed significantly higher ( p < 0.01) variant of Val/Val of Mn-SOD compared to control group. In workers with ADH3 variant, significantly higher levels of DNA damage ( p < 0.01) were observed than the control population with the same genotype. It could be concluded that enzymes involved in the metabolism of FA and oxidative stress controlling are two important effective parameters for DNA damage of peripheral blood lymphocytes in exposed people. These results are regarded as a pioneer investigation for the management of health risks assessment.
甲醛（FA）在人体外周血淋巴细胞的遗传损伤评估中得到了阳性结果。基因多态性在化学物质的毒性中起着特殊作用。对职业性接触FA的工人的血细胞中的DNA损伤进行了评估。本研究测试了酒精脱氢酶III（ADH3）和锰超氧化物歧化酶（Mn-SOD）基因多态性与外周血淋巴细胞DNA损伤的关联。对来自三聚氰胺餐具车间的54名工人和来自乳制品生产车间的34名工人的基因多态性进行了评估。根据年龄、吸烟情况、工作经历和社会经济地位对对照组和暴露人群进行了匹配。通过碱性彗星试验对外周血淋巴细胞中的DNA损伤进行了评估。使用聚合酶链反应-限制性片段长度多态性方法测定了所有工人的ADH3和Mn-SOD基因型。与对照组相比，暴露个体中淋巴细胞的DNA损伤明显更高（p < 0.001）。暴露组的检查显示，与对照组相比，Mn-SOD的Val/Val变异体明显更高（p < 0.01）。在具有ADH3变异体的工人中，观察到的DNA损伤水平明显高于具有相同基因型的对照组（p < 0.01）。可以得出结论，参与FA代谢和控制氧化应激的酶是暴露人群外周血淋巴细胞DNA损伤的两个重要有效参数。这些结果被视为健康风险评估管理的开创性研究。
