葡萄籽原花青素提取物对大鼠实验性糖尿病肾病的影响

[Effects of grape seed proanthocyanidins extracts on experimental diabetic nephropathy in rats].

作者信息

Liu Yan-ni, Shen Xin-nan, Yao Guo-ying

机构信息

Department of Nutrition and Food Hygiene, School of Public Health, Fudan University, Shanghai 200032, China.

出版信息

Wei Sheng Yan Jiu. 2006 Nov;35(6):703-5.

PMID:17290746

Abstract

OBJECTIVE

To study the effects of grape seed proanthocyanidins extracts(GSPE) and its mechanism on early renal lesions of diabetic rats.

METHODS

Diabetic rats induced by alloxan were given GSPE intragastrically for 6 weeks, then the antioxidative indexes and NO content, NOS activity in kidney and serum were measured, and the renal function indexes were tested as well.

RESULTS

Compared with the diabetic group, the urinary protein/24h, levels of blood urea nitrogen(BUN)and serum creatinine(SCr), creatinine clearance rate(CCr) and the ratio of kidney weight/body weight were decreased, the SOD activity in kidney was raised while MDA content was fall in the GSPE group(high dose), and the differences were all significant. The NO content in the kidney and NOS activity in kidney and serum decreased in the GSPE (low dose)group, and there was significant difference when compared with diabetic group( P <0.05) .

CONCLUSION

GSPE has the effect in protecting kidney of diabetic rats, the mechanism might be related with its action in increasing the renal antioxidative ability,decreasing the content of NO and the activity of NOS in kidney and serum.

摘要

目的

研究葡萄籽原花青素提取物（GSPE）对糖尿病大鼠早期肾脏病变的影响及其作用机制。

方法

用四氧嘧啶诱导糖尿病大鼠，给予GSPE灌胃6周，然后检测肾脏和血清中的抗氧化指标、NO含量、NOS活性，并检测肾功能指标。

结果

与糖尿病组相比，GSPE组（高剂量）24小时尿蛋白、血尿素氮（BUN）和血清肌酐（SCr）水平、肌酐清除率（CCr）及肾重/体重比值降低，肾脏SOD活性升高而MDA含量降低，差异均有统计学意义。GSPE组（低剂量）肾脏NO含量及肾脏和血清NOS活性降低，与糖尿病组比较差异有统计学意义（P<0.05）。